한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제26권5호
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- Pages.427-434
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- 1998
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Xanthomonas sp. YL-37 균주가 생산하는 Alkali성 단백질분해효소의 정제 및 성질
Purification and Properties of Alkaline Pretense from Xanthomonas sp. YL-37
초록
Xanthomonas sp. YL-37 이 생산하는 alkaline protease를 정제하기 위하여 ammonium sulfate로 침전시켜 회수하여 CM-cellulose ion exchange resin column에 주입한 후 Sephadex G-100 column에 2회 통과시켜 단일효소를 얻었으며 분자량은 약 62,000 dalton으로 단일 subunit로 되어있다. 정제효소의 반응최적온도는 5
An alkaline protease was 4-fold purified, yielding 2.3% of recovery by ammonium sulfate precipitation, CM-cellulose column chromatography and Sephadex G-100 column chromatography. The purified enzyme was estimated to be monomeric with molecular weight of about 62,000 from polyacrylamide gel eletrophoresis (PAGE) and sodiumdodecylsulfate polyacrylamide gel electrophoresis (SDS-FAGE). The optimal pH and temperature of the alkaline pretense activity were 11.0 and 50