한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제26권5호
  • /
  • Pages.393-399
  • /
  • 1998
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Phytophthora capsici의 성장을 저해하는 Enterobacter sp. B54의 선발과 Tn5 lac을 이용한 돌연변이 유기

Tn5 lac Mediated Mutagenesis of Enterobacter sp. B54 Antagonistic to Phytophthora capsici.

  • 윤상홍 (농업과학기술원 분자유전과) ;
  • 최청 (영남대학교 식품가공학과)
  • Yoon, Sang-Hong (Department of Molecular Genetics, National Institute of Agricultural Sciences, RDA) ;
  • Choi, Chung (Department of Food Science and Technology, Yeung Nam University)
  • 발행 : 1998.10.01
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초록

고추역병균(Phytophthora capsici)의 성장을 in vitro에서 저해하는 길항균 Bl4를 한국토양으로부터 분리 동정하여 Enterobacter속임을 밝혔고 Pl::Tn5 lac에 의해 transposon돌연변이를 유기하여 길항력 강화주와 약화주들의 염색체내에 Tn5 lac이 각기 상이한 위치에 무작위로 삽입되었음을 southern hybridization에 의해 확인하였다.

Enterobacter sp.B54 which shows antagonistic activity to Phytophthora capsici on potato dextrose agar was selected among 112 strains isolated from Korean soil. After Tn5 lac-induced mutants were obtained through Pl :: Tn5 lac mutagenesis, 2 mutants for loss of antibiosis and 1 mutant for increased antibiosis were screened by using in vitro fungal inhibition assay. When the 3 mutants affected in antibiosis were analyzed by southern hybridization with pRZ102 (ColEl :: Tn5) as a probe, its results suggest that Tn5 lac was randomly inserted into different chromosomal sites in these mutants.

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