Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Tn5 lac Mediated Mutagenesis of Enterobacter sp. B54 Antagonistic to Phytophthora capsici.

Phytophthora capsici의 성장을 저해하는 Enterobacter sp. B54의 선발과 Tn5 lac을 이용한 돌연변이 유기

  • Yoon, Sang-Hong (Department of Molecular Genetics, National Institute of Agricultural Sciences, RDA) ;
  • Choi, Chung (Department of Food Science and Technology, Yeung Nam University)
  • 윤상홍 (농업과학기술원 분자유전과) ;
  • 최청 (영남대학교 식품가공학과)
  • Published : 1998.10.01
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Abstract

Enterobacter sp.B54 which shows antagonistic activity to Phytophthora capsici on potato dextrose agar was selected among 112 strains isolated from Korean soil. After Tn5 lac-induced mutants were obtained through Pl :: Tn5 lac mutagenesis, 2 mutants for loss of antibiosis and 1 mutant for increased antibiosis were screened by using in vitro fungal inhibition assay. When the 3 mutants affected in antibiosis were analyzed by southern hybridization with pRZ102 (ColEl :: Tn5) as a probe, its results suggest that Tn5 lac was randomly inserted into different chromosomal sites in these mutants.

고추역병균(Phytophthora capsici)의 성장을 in vitro에서 저해하는 길항균 Bl4를 한국토양으로부터 분리 동정하여 Enterobacter속임을 밝혔고 Pl::Tn5 lac에 의해 transposon돌연변이를 유기하여 길항력 강화주와 약화주들의 염색체내에 Tn5 lac이 각기 상이한 위치에 무작위로 삽입되었음을 southern hybridization에 의해 확인하였다.

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