Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 26 Issue 4
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- Pages.365-368
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- 1998
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Optimization of Refolding Conditions for the Aklavinone 11-Hydroxylase of Streptomyces peucetius Overexpressed in Escherichia coli.
대장균에서 대량 발현된 Streptomyces peucetius유래 Aklavinone 11-Hydroxylase효소의 최적 가용화 조건
Abstract
The aklavinone 11-hydroxylase which was overexpressed using T7 promoter in E. coli could be detected in SDS-PAGE only in insoluble precipitate without any detectable enzyme activity. The insoluble enzyme was solubilized in 6M guanidine