Enterobacter cloacae MG82에 의한 Crystal Violet의 탈색특성

Decolorizing Characteristics of Crystal Violet by Enterobacter cloace MG82.

Triphenylmethane계 염료 탈색균인 E. cloacae MG 82를 이용한 crystal violet의 탈색 특성을 조사하였다. 배지내의 용존산소의 양이 많을수록 crystal violet의 탈색과 E. cloacae MG82의 균생장이 좋았다. 균생장 중기의 세포 군에서 crystal violet의 탈색능이 가장 활발하였고 염료의 농도가 높아짐에 따라 균생장과 탈색능이 억제되었으며, 균생장이 가능한 crystal violet의 최고 농도치는 375 $\mu$M이었다. E. cloacae MG82는 crystal violet을 유일한 탄소원으로 이용할 수 없었고, 균생장 및 염료의 탈색에 또 다른 에너지원을 필요로 하였다.

Decolorizing characteristics of crystal violet by Enterobacter cloace MG82, which can decolorize rapidly triphenylmethane dyes, were investigated. The higher growth and decolorization activity was shown at big ratio of dissolved oxygen in the medium. The decolorization activity of crystal violet revealed highest at the middle of lag phase. As the concentration of crystal violet was higher, the growth of E. cloacae MG82 and decolorizing activity of crystal violet by this strain were worse. The maximum concentration of crystal violet at which E. cloacae MG82 be able to grow was 375 ${\mu}$M. E. cloacae MG82 was not able to use the crystal violet itself as a sole carbon source. So, it was shown that growth of E. cloacae MG82 and decolorization activity of crystal violet by this strain needed addition of another energy sources except this dye.