Journal of Microbiology and Biotechnology
- 제8권6호
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- Pages.631-638
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- 1998
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Characterization of Cell Growth and Camptothecin Production in Cell Cultures of Camptotheca acuminata
- Song, Seung-Hoon (School of Chemical Engineering and Biotechnology, Ajou University) ;
- Byun, Sang-Yo (School of Chemical Engineering and Biotechnology, Ajou University)
- 발행 : 1998.12.01
초록
Studies were made to elucidate the cell growth and the production of camptothecin and its derivatives in cell cultures of Camptotheca acuminata. High resolution HPLC chromatograms to analyze camptothecin and 10-hydroxycamptothecin in lactone and carboxylate forms were obtained with a fluorescence detector. Calli inductions were optimized with the young stem of explant on Schenk and Hildebrandt (SH) medium supplemented with 5 mg/l