Journal of Microbiology and Biotechnology
- Volume 8 Issue 5
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- Pages.461-465
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- 1998
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Suspension Culture of an Antibacterial Peptide Producing Cell Line from Bombina orientalis
- KIM, YONG-HWAN (Bioprocess and Biomaterials Engineering Laboratory, Graduate School of Biotechnology, Korea University) ;
- JAE-WON YANG (Bioprocess and Biomaterials Engineering Laboratory, Graduate School of Biotechnology, Korea University) ;
- CHAN-WHA KIM (Bioprocess and Biomaterials Engineering Laboratory, Graduate School of Biotechnology, Korea University)
- Published : 1998.10.01
Abstract
The suspension culture of an anchorage-dependent cell line (Bok-l) from Bombina orientalis was successful in respects of cost and efficiency. The amount of cells obtained from the suspension culture was almost equivalent to that from the anchorage-dependent culture. This result shows the possibility of suspension culture for scale-up. The cells in suspension produced an antibacterial peptide as much as anchorage-dependent cells did. The cell growth (