Journal of Microbiology and Biotechnology
- Volume 8 Issue 4
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- Pages.353-360
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- 1998
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Production and Characterization of Acid-stable Pectin Lyase from Bacillus sp. PN33
- Kim, Jong-Chon (Graduate School of Biotechnology, Korea University) ;
- Kim, Hwa-Young (Graduate School of Biotechnology, Korea University) ;
- Choi, Yong-Jin (Graduate School of Biotechnology, Korea University)
- Published : 1998.08.01
Abstract
A bacterial strain PN33 producing large amounts of extracellular pectin lyase (PNL, EC 4.2.2.10) was isolated from soil. The isolated bacterium was identified as a strain of Bacillus sp. Production of PNL by the strain was induced only by pectins, with a higher degree of esterification, which had been added to the culture medium as a sole carbon source. The optimal medium for PNL production was determined to consist of 10 g pectin, 2 g yeast extract, 4 g