Journal of Microbiology and Biotechnology
- 제8권3호
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- Pages.251-257
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- 1998
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Purification and Characterization of Cycloinulooligosaccharide Fructanotransferase from Bacillus macerans CFC1
- Kim, Hwa-Young (Graduate School of Biotechnology, Korea University) ;
- Choi, Yong-Jin (Graduate School of Biotechnology, Korea University)
- 발행 : 1998.06.01
초록
Cycloinulooligosaccharide fructanotransferase (CFTase) which produces cyclofructan from inulin was purified 332-fold from a culture broth of Bacillus macerans CFCl. The molecular mass of the CFTase was estimated to be 110 kDa by SDS-polyacrylamide gel electrophoresis and gel filtration, indicating that the enzyme has a monomer structure. The maximal level of enzyme activity was observed at pH 7.5 and
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