Antifungal Activity of Medium-Chain ($C_{6}-C_{13}$) Alkenals against, and Their Inhibitory Effect on the Plasma Membrane $H^{+}$-ATPase of Saccharomyces cerevisiae

  • Lee, Jae-Ran (Department of Microbiology, Kyung-pook National University) ;
  • Lee, Sang-Hwa (Department of Environmental Science, Policy and Management, University of California) ;
  • Kubo, Isao (Department of Environmental Science, Policy and Management, University of California) ;
  • Hong, Soon-Duck (Department of Microbiology, Kyung-pook National University)
  • Published : 1998.06.01

Abstract

Aliphatic alkenals having 6 to 13 carbons were evaluated for antifungal activity against Saccharomyces cerevisiae. The activity was gradually increased with chain length, e.g., (E)-2-decenal and (E)-2-undecenal exhibited maximum potency, while (E)-2-dodecenal and (E)-2-tridecenal were completely inactive. Alkenals showed increasing inhibitory activity with chain length, as in the case of antifungal activity, towards glucose-induced medium acidification by the plasma membrane $H^+$-ATPase of S. cerevisiae. The group including (E)-2-nonenal, (E)-2-decenal, and (E)-2-undecenal exhibited maximum potency, but the potency of (E)-2-dodecenal and (E)-2-tridecenal demonstrated a sudden drop with respect to the former group. (E)-2-Nonenal revealed dose-responsive inhibition to the medium acidification and inhibited over 90% at a concentration of 1.25 mM ($175.3{\mu}g$/ml). In contrast to (E)-2-undecenal whose inhibitory efficiency increased with incubation time, inhibition by (E)-2-dodecenal was reversed with time. Of the tested alkenals, (E)-2-heptenal and (E)-2-octenal most highly inhibited ATP hydrolytic activity by the plasma membrane $H^+$ ATPase, while (E)-2-heptenal at 10 mM ($1121.8{\mu}g$/ml) showed an inhibitory efficacy of 93.2%.

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