Journal of Microbiology and Biotechnology
- Volume 8 Issue 2
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- Pages.152-157
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- 1998
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Effect of C- or D-Domain Deletion on Enzymatic Properties of Cyclodextrin Glucanotransferase from Bacillus stearothermophilus NO2
- Jeon, Sung-Jong (Department of Microbiology, Dong-Eui University) ;
- Nam, Soo-Wan (Department of Microbiology, Dong-Eui University) ;
- Yun, Jong-Won (Department of Biotechnology, Taegu University) ;
- Song, Seung-Koo (Department of Chemical Engineering, Pusan National University) ;
- Kim, Byung-Woo (Department of Microbiology, Dong-Eui University)
- Published : 1998.04.01
Abstract
To analyze the role of the C and D domains in the cyclization activity of cyclodextrin glucanotransferase (CGTase), two plasmids, pKB1ΔC300 and pKB1ΔD96, were constructed in which DNA regions encoding 100 and 32 amino acids, respectively, from the C and D domains of B. stearothermophilus NO2 CGTase were deleted. The mutated CGTase from the pKBlΔC300 produced much lower amounts of