Journal of Microbiology and Biotechnology
- Volume 8 Issue 2
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- Pages.111-118
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- 1998
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Purification and Characterization of Antistaphylococcal Substance from Pseudomonas sp. KUH-001
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Hwang, Se-Young
(Graduate School of Biotechnology, Korea University) ;
- Lee, So-Hee (Graduate School of Biotechnology, Korea University) ;
- Song, Kook-Jong (Graduate School of Biotechnology, Korea University) ;
- Kim, Yong-Pil (Graduate School of Biotechnology, Korea University) ;
- Kawahara, Kazuyoshi (Department of Bacteriology, The Kitasato Institute)
- Published : 1998.04.01
Abstract
A bacterium producing unique antistaphylococcal substance (ASS) was isolated from soil samples. The isolated strain KUH-001 was identified to belong to Pseudomonas species from the characteristic properties of its fluorescence and cellular 3-hydroxy fatty acid composition, etc. The ASS component was purified by procedures employing activated carbon adsorption, column chromatography with silica gel, preparative TLC and HPLC. This compound could also be purified mainly by repeating of trituration and precipitation with chilled ether. Purified ASS with a m.p. value of