Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inhibition of DNA Topoisomerase I by Cryptotanshinone from Salvia miltiorrhiza

  • Lee, Dong-Sun (Department of Microbiology, College of Natural Science, Kyungpook National University) ;
  • Hong, Soon-Duck (Department of Microbiology, College of Natural Science, Kyungpook National University)
  • Published : 1998.02.01
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Abstract

Cryptotanshinone induced topoisomerase I-mediated DNA cleavage in vitro as strongly as camptothecin, whereas topoisomerase II-mediated DNA cleavage was not induced by this agent. In DNA relaxation assay using calf thymus DNA topoisomerase I and supercoiled pBR322 DNA, cryptotanshinone inhibited topoisomerase I-mediated DNA relaxation in a dose-dependent manner. In unwinding assay, cryptotanshinone ($50{\mu}M$) did not shift the topoisomers of DNA. These results suggest that cryptotanshinone exerted a preferential inhibition of topoisomerase I without intercalating into DNA.

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