Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of Multiple Active Forms in Cellulase-xylanase of Aspergillus sp. 8-17 by Active Staining

  • Shin, Pyung-Gyun (Horticulture Division, National Alpine Agricultural Experiment Station, RDA) ;
  • Ahn, Jun-Bae (Department of Food Technology, Seoul National University) ;
  • Kim, Chang-Young (Crop Division, Gyeongnam Provincial RDA) ;
  • Jeong, Won-Hwa (Boryung Biopharma Co., LTD) ;
  • Ryu, Jin-Chang (Molecular Genetics Division, National Agricultural Science and Technology Institute)
  • Published : 1998.02.01
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Abstract

A fungal strain able to produce filter paper activity (FPase) was isolated from soil by testing the ability to hydrolyze using filter paper. The isolated strain was identified as an Aspergilus sp. judging from its morphological and microscopical characteristics. The cellulase-xylanase system of Aspergillus sp. 8-17 was detected in situ after gel electrophoresis in the presence of SDS and showed that each protein pattern had a distinct polypeptide composition. ${\beta}$-1,4-Glucanase, cellobiohydrolase, and xylanase activity profiles differ from protein patterns. The Aspergillus sp. 8-17 hydrolytic enzymes responsible for the hydrolysis of ${\beta}$-glucan, MUC, and xylan have multiple active forms.

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