Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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An Electron Microscopic Study on the Cell Wall Regeneration of Culture Panax ginseng Callus Protoplast

인삼 캘러스 원형질체의 배양에 따른 세포벽 재생의 전자현미경적 연구

  • 박종범 (신라대학교 자연과학대학 생물학과)
  • Published : 1998.11.01
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Abstract

Ultrastructural changes of the isolated and cultured protoplasts from ginseng (Panax ginseng C. A. Meyer) callus were studies with electron microscopy. In the 3-day-cultured protoplasts, the cell organelles such as rough endoplasmic reticulum, ribosome, Golgi complex, mitochondria, proplastid increased in number and observed microtubules. Many vesicles derived from the Golgi complex were evenly distributed in the cytoplasm. Some of such vesicles protruded the outer surface of the plasmalemma, and formed the protuberances. Vacuole derived from endoplasmic reticulum included Golgi vesicles by the invagination of vacuoles. These vacuoles migrated toward the plasmalemma by a fusion process (exocytosis), after fusing the plasmalemma the cell wall materials released from the outer surface of the plasmalemma, and lastly deposited on the plasmalemma. Proplastids containing many starch grains, and microtubules parallel to the plasmalemma were observed near the plasmalemma. Connected fibrils which were observed on the outer surface of the 3-day-cultured protoplast were interpreted as the component of cellulose.

인삼(Panax ginseng C. A. Meyer) 캘러스로부터 분리한 원형질체를 배양하면서 배양시간에 따른 원형질체의 미세구조 변화와 원형질막 표면을 전자현미경으로 조사하였다. 3일 동안 배양된 원형질체에서는 조면소포체, 리보좀, 골기체, 미토콘드리아, 전색소체 등의 세포기관들의 수가 증가하였고 미소관도 관찰되었다. 골기체 주변에는 많은 골기소낭들이 형성되어 세포질 전반에 존재하였고, 이들 소낭들은 원형질막 바깥으로 돌출되어 돌기를 형성하기도 하였다. 소포체에서 유래된 액포속에는 액포의 함입에 의하여 골기소낭들이 들어 있는데, 이들 액포들은 융합에 의한 exocytosis로 원형질막 근처로 이동하여 원형질막과 융합한 다음, 세포벽 물질을 원형질막 바깥으로 방출하여 원형질막에 침적하였다. 전색소체는 많은 전분립을 함유하고 있었고, 미소관들은 원형질막 근처에서 막과 평형으로 배열하고 있었다. 배양된 원형질체의 표면에는 섬유소로 구성되어 있는 원섬유들이 서로 연결되어 있는 것이 관찰되었다.

Keywords

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