Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Enhanced Development and Germination of Carrot Somatic Embryos on Modified Surface of Medium

당근체세포배의 발생 및 발아에 미치는 배지표면의 물리적변화

  • Published : 1998.07.01
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Abstract

Carrot cotyledon explants cultured on MS medium with 1 mg/L 2,4-D were transferred to a hormone-free solid medium overlaid with filter paper in order to elucidate the effect of simple physical treatment on the development and germination of somatic embryos. Transfer of the explants cultured for one week on MS basal medium overlaid with 3 sheets of filter paper on to MS basal medium increased somatic embryo production 2-39 times over the one week culture on medium without filter paper overlay. Maturation and germination of somatic embryos was more prominent on medium overlaid with filter paper than on medium without filter paper. The explants cultured for one week on filter paper overlay added with liquid medium showed prominent decrease in somatic embryo formation compared to filter paper overlay only. It is suggested that the filter paper overlay affected the moisture environment of the somatic embryos developing on it.

배지상에 여과지를 덮어놓는 간단한 물리적 처리가 체세포배의 발생 및 발아에 미치는 영향을 밝히기 위하여 본 실험관찰을 수행하였다. 배지상에 덮어놓은 세장의 여과지위에서 일주일 동안 당근 자엽절편을 배양한 후 MS 기본배지에 계대배양을 하면 여과지 덮개 없는 배지에서의 체세포배발생 보다 2배에서 39배의 배발생을 나타내었고, 체세포배의 성숙 및 발아도 증진되었다. 배지상의 여과지에 배양액을 첨가한 다음 배양을 하면 여과지에 배양액을 첨가하지 않은 경우보다 배발생이 감소되었다. 그러므로 여과지덮개는 자엽절편을 치상한 배양환경의 습기를 변화시켜 체세포배의 발생을 증진시킨 것으로 생각된다.

Keywords

References

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