Diversity of Anther-derived Plants in Hot pepper (Capsicum annuum L.)

고추 약배양 후대식물체의 다양성

  • Published : 1998.05.01

Abstract

Anthers of three cross combinations of hot-pepper(Capsicum annuum L.) were cultured on Dumas De Vaulx medium supplemented with some growth regulators. The embryo production efficiency and the diversity for agronomic traits in $A_2$ lines were investigated. The embryo production frequencies of hybrid combinations were ranged from 16.4% to 43.4%, the highest embryo induction combination was DGSH $\times$ C-NH with 43.4% embryogenic efficiency. Among total 275 $A_2$ lines, phenotypic variants were found in six lines, 2.1% variant frequency. The diversity of $A_2$ lines derived from anther culture was different according to the cross combinations. Fruit color was within parental range, no transgressive variation was observed. However leaf color showed transgressive variation. In fruit length, fruit width and fruit weight, one C-HC $\times$ DGSH and DGSH $\times$ C-NH showed great diversity compared with doner parents while Cheokjo 1 $\times$ C-NH crossed with Cheokjo 1 with big fruit shape showed small diversity. Stem length to 1st branch was relatively similer to or longer than donor parents. Stem thick exhibited remarkable diversity. Node number to 1st branch distributed alomost within the range of donor parents in C-HC $\times$ DGSH combination, however great transgressive variations were observed in DGSH $\times$ C-NH and Cheokjo 1 $\times$ C-NH combinations.

고추 교잡계통의 약배양 후대를 재료로 하여 배상체 발생률과 주요 농업형질에 대한 다양성을 조사하였다. 교배조합간 약배양 효율이 16.4%부터 43.4%까지 분포하였고 교배조합중 약배양 호율이 높은 계통 DGSH와 교배된 DGSH$\times$C-NH 조합에서 배 발생율이 43.4%로 가장 높았다. 3개 교배조합에서 얻은 약배양 후대 275개 $A_2$계통 중 계통내 개체별 분리를 보이는 계통은 총 6계통으로 2.1%를 차지하였다. $A_2$ 계통들의 형태적 다양성을 보면 청과색은 양친이 녹색과인 것이 교배된 조합에서는 녹색과만, 그리고 녹색과와 농녹과가 교배된 조합의 후대에서는 녹색과와 농녹과 계통만 출현하였다. 엽색에서도 조합에 따라 출현 빈도는 다르지만 녹색잎과 농녹색 잎의 발현 현상은 청과색과 비슷하였지만 모친에 없는 엽색도 소수 나타났다. 과장, 과경, 1과중은 C-HC $\times$DGSH, DGSH $\times$C-NH조합에서 얻어진 $A_2$ 약배양 계통들에서 양친보다 다양한 변이양상을 보인 계통들이 많이 출현하였으나 과장, 과경, 1과중이 현저하게 큰 적조 1호가 교배된 적조 1호$\times$C-HC 조합에서 얻어진 약배양 후대 계통들은 다양성이 낮았다. 1차 분지점까지의 줄기 길이는 3개 조합 공히 양친과 비슷한 것들이 많이 출현하였고 양친보다 긴 것도 다수 출현하였다. 주경의 굵기는 양친들의 주경 굵기보다 적거나 큰 것들이 다수 출현하여 큰 다양성을 보였다. 1차 분지점까지 마디수는 C-HC$\times$DGSH 조합에서 양친과 비슷한 것이 가장 많았으나, DGSH$\times$C-NH 조합과 적조 1호$\times$C-NH 조합에서는 양친의 마디수 보다 적거나 많은 것들도 많이 출현하여 큰 다양성을 보였다.

Keywords

References

  1. Handbook of plant cell culture v.1 In vitro production of haploids Bajaj YPS;Evans(ed.)
  2. Tissue and Organ Culture v.46 Effect of genotype, explant and medium on in vitro regeneration of red pepper:Plant Cell Christopher T;Rajam MV
  3. Agronomie v.1 Culture in vitro d,anteres de piment (Capsicum annuum L.): Amelioration des taux d,obtention de plantes chez differents genotypes par des traitments at 35℃ Dumas De Vaulz RD;Chambonnet D;Pchard E
  4. Nature v.204 In vitro production of embryos from anther of Datura Guha S;Maheshwari SC
  5. Nature v.212 Cell division and differntitation of embryos in the pollen grains of Datura in vitro Guha S;Maheshwari SC
  6. Korean J Plant Tissue Culture v.3 Studies on the anther culture of Capsicum annuum Ham CR;Kim MZ;Choi KJ;Lee YI
  7. Theor Appl Genet v.55 Stimulation of embryogenesis and haploid production in Brassica campestris anther culture by elevated temperature treatment Keller WA;Armstrong KC
  8. Korean J Plant Tissue Culture v.12 Basic studies on the induction of microspore-originated calluses of embryos in the anther culture of Capsicum annuum L. Kim MZ;Kim YR
  9. Korean J Breed v.25 Genotypic effect on callus formation in anther culture of barley LeeJH;Baek SB;Lee MS
  10. Korean J Breed v.20 Production of plants through anther culture and their seed bearing ability in chinese cabbige Lee SS;Yoon YJ;Shin HH;Woo JG
  11. Korean J Breed v.27 Some characteristics and electrophoretic patterns of seed protein rice variants derived from tissue culture Lee Sy;Lee JK;Kang HJ;Shin HT;Lee SY
  12. Czern. Plant Cell, Tissue and Organ Culture v.4 Micropsore embryogenesis in anther culture of two inidian cultivars of Brassica juncea(L.). Sharma KK;Bhojwani SB
  13. Korean J Breed v.27 Variation of some agronomic traits in anther-derived rice plants Sohn JK;Yi GH;Oh BG;Lim SJ
  14. Japan J Breed v.39 Varietal differences in embryogenic ability in anther of chinese cabbage (Brassica campestris ssp. pekinesis) Takanori S;Takeshi N;Masashi H
  15. Sci Sin v.16 The induction of the pollen plantlets of triticale and Capsicum annum from anther culture Wang YY;Sun CS;Wang CC;Chien NF
  16. Korean J Breed v.20 Variability of anther-derived plantlets in rice (Oryza sativa L.) Yang SJ;Oh BG;Chung GS;Sohn JK
  17. Korean J Breed v.21 Variability of anther-derived plantlets in rice (Oryza sativa L.).Ⅳ. Clssification of plants derived from Tongil/Japonica F₁hybrid pollen Yang SJ;Oh BG;Lee Sk;Chung GS
  18. J Korean Soc Sci v.32 Plant induction by anther culture of hot-pepper(Capsicum annuum L.) Yoon YJ;Lee KS;Chang SK