Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Tissue Specific Expression of Tomato Phenylalanine Ammonia-lyase Gene in Transgenic Tobacco Plants

형질전환 담배에서 토마토 PAL유전자의 조직 특이적 발현

  • YI, Jung-Yoon (Dep. of Horticultural Science, Korea University) ;
  • Lee, Shin-Woo (National Agricultural Sciences and Technology Institute) ;
  • SEO, Hyo-Won (National Alpine Agricultural Experiment Station, RDA) ;
  • PARK, Kuen-Woo (Dep. of Horticultural Science, Korea University)
  • Published : 1998.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Tomato phenylalanine ammonia-lyase 5 (tPAL5) was identified that alternate initiation sites were utilized differentially in response to environmental stimuli (Lee et al, 1992b). In this study, we tried to look into tissue -or cell- specific expression pattern of tPAL5 gene by fusing with ${\beta}-glucuronidase$ (GUS) gene in transgenic tobacco plants. In transgenic plants, root and stem extracts contained 8~12 fold higher levels of GUS activity than petiole or leaf tissue while the highest levels of induction was observed from leaf tissue by mechanical wounding (5~11 fold). In trans-sections of stems and petioles, GUS activity was restricted to phloem cells(outer region) of developing vascular bundle and mainly at apical tip region in the root tissues. The levels of GUS activity was drastically reduced (10~12 fold reduction) when the 5'-upstream region of tPAL5 gene (-1151bp from ATG codon) was deleted up to -665. The levels of GUS expression, however, raised up by 6~8 fold when deleted up to -455. Therefore, we conclude that there are positive cis-elements at the region -1151 to -1008 and at -455 to -195 while the negative cis-element is at -1008 to -455.

Keywords

References

  1. EMBO J v.4 New cloning vechicle for transformation of higher plants An G;Watson BD;Stachel S;Gordon MP;Nester EW
  2. Plant Mol Biol Man v.A3 Binary vectors An G;Ebert PR;Miltra A;Ha SB
  3. Plant Mol Biol. v.12 Phenylalanine ammonia-lyase gene organization and structure Cramer CL;Edwards K;Dron M;Liang X;Dildine SL;Bolwell GP
  4. Ann Rev Plant Physiol v.30 Enzymatic controls in the biosynthesis of lignin and flavonoids Hahlbrock K.;Grisebach H
  5. Cell v.56 Signals and transduction mechanisms for activation of plant defenses against microbial attack Lamb CJ;Lawto MA;Dron M;Dixon RA
  6. Plant Mol Biol v.18 Reduced PAL gene suppression in Verticillum-infected tomatoes Lee SW;Nazar RN;Powell DA;Robb J
  7. J. Biol Chem. v.267 Truncated phenylalanine ammonia-Iyase expression in tomato Lee SW;Robb J;Nazar RN
  8. Eur J Biochem. v.226 Differential utilizaiton of alternate initiation sites in a plant defense gene responding to environmental stimuli Lee SW;Heinz R;Robb J;Nazar RN
  9. J. Biol Chem v.264 Differenetial regulation of phenylalanine ammonia-Iyase genes during plant development and enviromental cues Liang X;Dron M;Schmid J;Dixon RA;Lamb CJ
  10. Proc Natl Acad Sci USA v.86 Developmental environmental regulation of a phenylalanine ammonia-Iyase-β-glucuronidase gene fusion in transgenic tobacco plants Liang X;Dron M;Cramer CL;Dixon RA:Lamb CJ
  11. EMBO J v.8 A phenylalanine ammonia-lyase gene from parsley:structure, regulation, and identification of elicitor and light response cis acting elements Lois R;Dietrich A;Hahlbrock K;Schlz W
  12. Mol Gen Genet v.210 Organization and differential activation of a gene family encoding the plant defense enzyme chalcone synthase in Phaseolus vlgaris Ryder TB;Hedric SA;Bell JN;Liang X;Clouse SD;Lamb CJ