펄프종이기술 (Journal of Korea Technical Association of The Pulp and Paper Industry)

한국펄프종이공학회 (Korea Technical Association of the Pulp and Paper Industry)
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제지 공정 적용을 위한 Cellulase의 정제 특성 및 처리 조건 선정에 관한 연구

Purification Properties and Treatment Conditions of Cellulase for Papermaking Processes

  • 김병현 (중부대학교 공과대학 인쇄공학과) ;
  • 전양 (충남대학교 농과대학 임산공학과)
  • Kim, Byung-Hyon (Dept. of Grafic Art TechNology, College of Engineering, Joong Bu University) ;
  • Jeon, Yang (Dept. of Forest Product TechNology, College of Agriculture, Chungnam National University)
  • 발행 : 1998.03.01
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초록

The main protein peak was observed in fraction No.9 and 109 when cellulase seperation was conducted by use of DEAE-Sephadex. The protein obtained from fraction No.9 has the characteristics of Cx component and that from fraction No.109 characteristics similiar to $C_1$ component. The effective reaction condition of the ensyme used was $40^{\circ}C$ in temperature. pH 5.0 and 90 minutes in treatment time. For the case of $C_1$ pH 5.5 in temperature range of $30^{\circ}C 50^^{\circ}C$, 4.0 5.5 in pH, and over 30 minutes of treatment time, the reaction was in the range of 80% of the maximum. Affinity of enzyme increased as freeness, increased, and this effect was more visible in fiber than in fines.

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참고문헌

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