Isolation and Characterization of Bacillus sp. BT182-3 for Biocontrol Against a Plant Pathogenic Pseudomonas syringae

식물병원균 Pseudomonas syringae에 대한 생물방제균 Bacillus sp. BT182-3의 분리 및 특성

  • 김광현 (동의대학교 미생물학과) ;
  • 김위종 (동의대학교 미생물학과) ;
  • 이광배 (대구보건대학 보건위생과)
  • Published : 1998.12.01

Abstract

For a microbial control of a plant pathogenic Pseudomonas syringae, Bacillus sp. strain BT182-3 was isolated. The strain BT182-3 had a growth inhibition against P. syringae not only on agar plate but also on cultured broth. After heat treatment at $40^{\cird}C$ and $80^{\cird}C$ for 30min, the lytic substance from the strain BT182-3 had about 52% remaining activity and 17% remaining activity, respectively. The optimal pH and temperature of the lytic substance was 6.0 and $28^{\cird}C$, respectively. Germination ratio of healthy radish seeds was 87% at $25^{\cird}C$ for 5 days in 0.8% saline, and that of the radish seeds infected with P. syringae was 67%, while that of the radish seeds treated with cultured broth of the strain BT182-3 was 90%. The 5-days healthy radish seedlings were 3.90cm at high and the seedlings infected with P. syringae were 3.06cm at high, while the seedlings treated with cultured broth of the strain BT182-3 were 4.30cm at high. The growth of the radish seedlings infected with P. syringae was inhibited after cultivation for 40days on pots, while the growth of the infected radish seedlings with P. syringae was recovered at stem length, root length and total weight at the same as the healthy seedlings after treatment of a lytic substance from the strain BT182-3.

Keywords