Optimal Storage Conditions of the Entomopathogenic Nematode, Steinernema Carpocapsae

곤충병원선충(Steinernema carpocapsae)의 최적보관을 위한 환경요인

  • 박영진 (안동대학교 자연과학대학 생명자원과학부 농생물학전공) ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학부 농생물학전공) ;
  • 이영근 (안동대학교 자연과학대학 생명자원과학부 농생물학전공) ;
  • 한상천 (안동대학교 자연과학대학 생명자원과학부 농생물학전공)
  • Published : 1998.06.01

Abstract

Effects of temperature, pH, and salt concentration on the preservation of the infective juvenile entolopathogenic nematodes, Steinernema carpocapsae, were analyzed. The effects of acclimation to sublethal low temperature and glycerol concentrations on rapid cold hardening of the nematodes were also evaluated to set up the conditions for their cryopreservation. All tested environmental factors could change the storage effectiveness of the nematodes. These analyses indicated that the optinal storage condition for the nematodes consisted of $15^{\circ}C$, pH 8.5, and 0.5-1.0% salt concentration. The nematodes showed a rapid could hardening in response to $5^{\circ}C$ for 2h. Glycerol induced the nematodes to increase the cold hardiness. Its high concentration over 10%, however, gave a harmful effect on the nematode survival.

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