Plant Resources
- Volume 1 Issue 1
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- Pages.13-21
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- 1998
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- 1226-8771(pISSN)
Isolation and Characterization of a Cdna ( Fp 1 ) Encoding the Iron Storage Protein in Red Pepper ( Capsicum annuum L. )
- Kim, Ho-Young (Dept. of Horticulture, Ansung National Univ.) ;
- Lee, Young-Ok (Dept. of Horticulture, Ansung National Univ.) ;
- Noh, Ill-Sup (Dept. of Horticulture, Sunchon National Univ.) ;
- Kang, Hee-Wan (National Institute Agriculture Sci. Technology, RAD) ;
- Kameya, Toshiaki (Institute of Genetic Ecology, Tohoku Univ.) ;
- Saito, Takashi (Dept. of Horticulture, Tokyo Univ. of Agriculture) ;
- Kang, Kwon-Kyoo (Dept. of Horticulture, Ansung National Univ.)
- Published : 1998.04.01
Abstract
A cDNA Fragment encoding iron storage protrin generated by polymerase chain reaction(PCR) using highly conserved regions of ferritin related genes were used to sereen a red pepper cDNA library. cDNA clone was designated as Fp1. Fp1 clone contatines a 5' nontranslated region of 51dp containing stop conds. Down stream from 5' UTP. an open reading frame of 750bp was observed. followed by a 3' UTR of 272bp. The deduces amino acid sequence of red pepper protein(Fp1) showed 84%, 48% and 36% identity with soybean(SolC). human(HuL H) and horse spleen(HoS-L) ferritin mRNA accumulation in response to iron. Ferritin mRNA accumulation was transient and particularly abundant in leaves. reaching a maxmum at 12h. The level of ferritin mRNA in roots was affected to a lesser extent than in leaves.
Keywords
- alignment;
- mino acid spquence;
- DNA library;
- pl gene;
- iron storage protein;
- Northern blot.Southern blot