Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Immune Response of Alpha-toxin, Capsular Polysaccharide (CPS) and Recombinant Fibronectin-Binding Protein (r-FnBP) of Staphylococcus aureus in Rabbit

황색포도상구균의 Alpha-toxin, Capsular Polysaccharide (CPS)와 재조합 Fibronectin-Binding Protein (r-FnBP) 항원을 이용한 토끼에서의 면역반응

  • Park, Hee-Myung (Department of Internal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Yoo, Han-Sang (Department of Internal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Han, Hong-Ryul (Department of Internal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Doo (Kangwon National University)
  • 박희명 (서울대학교 수의과대학 내과학교실) ;
  • 유한상 (서울대학교 수의과대학 내과학교실) ;
  • 한흥율 (서울대학교 수의과대학 내과학교실) ;
  • 김두 (강원대학교 내과학교실)
  • Published : 1998.06.01
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Abstract

본 연구는 황색포도상구균의 병원성 인자인 alpha-toxin, casular polysaccharides (CPS)와 fibronectin-binding protein (FnBP)을 이용해 실험동물인 토끼에서의 항체가 형성 능과 공격접종 후 방어능에 관한 연구를 위하여 수행되었으며 향후 젖소 유방염 아단위 항원 을 이용한 백신개발 가능성을 탐색하고자 수행되어졌다. 황색포도상구균의 alpha-toxin, capsu18r polysaccharides (CPS)91 fibronectin-binding protein (FnBP) 항원을 이통재 효소면 역측정법을 통한 혈중 IgG 항체가수준을 측정하였으며 alpha-toxin, capsular polysaccharides (CPS)와 fibronectin-binding protein (FnBP)으로 면역시킨 토끼에서 대조군의 토끼보다 혈 중 항체가 수준이 1차 면역 이후 유의성 있게 높았다 (p<0.05). 백신에 사용된 alpha-toxin, capsular polysaccharides (CPS)와 fibronectin-binding protein (FnBP) 항원중 capsular polysaccharides (CPS)가 다른 alpha toxin과 fibronectin-binding protein (FnBP)의 혈중항 체가 수준과 비교하여 볼 때 비교적 낮은 수준이었다. 세균을 혈중으로 공격접종한 후 대조군 과 백신접종군의 혈중내 세균제거율에 있어 대조군에 비해 백신접종군에서 유의성 있게 낮은 균수를 보였다 (p<0.05). 또한 장기내 균수측정실험결과 대조군의 장기보다 백신접종군에서 유의성 있게 세균수가 낮게 출현하였다 (p<0.05). 결론적으로 본 연구결과 황색포도상구균의 3가지 항훤 alpha-toxin, capsular polysaccharide와 재조합 fibronectin binding protein을 이 퐁한 실험동물에서 안단위 유방염 백신은 방어능이 있다고 생각된다.

Keywords

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