화랑곡나방의 발생에 따른 Esterase Isozymes의 Pattern변화

Changes of Esterase Isozymes During the Development from Plodia interpunctella (Hiibner)


초록

Changes in activity and classification of esterase isozymes during the tire cycle or Plodia inteipunctella (Hiibner) were investigated by the native polyacrylamide gel electrophoresis. The stage specificity in esterase activity and isozyme pattern was observed throughout the larvalpupal-adult transformation. The activity esterase was highest at the 3-day old adult stage, and the lowest level at the prepupal stage. A total of 12 esterase bands were identified throughout the development, and the bands showing high enzyme activity was observed in the middle part of gel. Twelve esterases on the basis of inhibition by the three types of inhibitors (organophosphates, eserine sulfate and sulfhydryl reagents) were classified into three class, namely, carboxylesterase (CE), arylesterase (ArE) and cholinesterase (ChE), and these classes contained 7, 3 and 2 isozymes, respectively.

키워드

참고문헌

  1. Insect Biochem v.6 Larbal and adult gousefly carboxylesterase: Isozymatic composition and tissue pattern Ahmad, S.
  2. Drug. Metab. Rev v.5 Nonxidative enzymes in the metabolism of insecticides Ahmad, S.;A. J. Forgash
  3. Experimentra v.28 Nonspecific esterases in females of aedes aegypti(L.) Brigel, H.
  4. Biochem. J. v.167 The properties of a carboxylesterase from the peach-potato-aphid, Myzuspersicae(Sulz.), and its role in conferring insecticide resistance Devonshire, A. L.
  5. J. Insect Physiol v.11 Changes in esterase zymograms in the silkworm, Bombyx mori L., during development Eguchi, M.;T. Sugimoto
  6. J. Histochem. Cytochem v.16 Nonspecific esterases in mosquitos Freyvogel, T. A.;R. L. Hunter;E. M. Smith
  7. Insect Biochem v.3 Changes in soluble protein, dehydrogenases, and esterases of unfertilized and fertilized eggs of Musca domestica Gadallah, A. I.;N. Marei
  8. Comp. Biochem. Physiol v.49B The distribution of acetylcholine and unspecific esterases in the midgut of femal Aedes aegypti L Geering, K.;T. A. Freyvogel
  9. Appl. Entomol. Zool v.18 Electrophoretic esterase patterns in the brown planthopper Nilaparavata lugens Stal(Hemiptera: Delphacidae) shich developed resistance to insecticides Hideaki, H.;K. Ozaki
  10. Insect Biochem v.17 A time-internal activation of esterase $A_4$ by cold: Relation to the terminationof embryonic diapause in the silkworm, Bombyx morri Kai, H.;T. Kawai;Y. Kawai
  11. Insect Biochem v.10 Esterases in larval tissues of gypsy moth, Lymantria dispar(L.); Optimum assay conditions, quantification and characterization Kapin, M. A.;S. Ahmad
  12. Biochem. Pharmac v.31 Idenrification of chloramphenicol hydrolyzing enzyme of guinea pig liver as one of the nonspecific carboxylesterase Kuhn D.;E. Heyman
  13. Nature v.227 Cleavage of structural proteins during assembly of the head of bacterophage $T_4$ Laemmli, U. K.
  14. Ann. W. Y. Acad. Sci v.94 From of enzymes in insect development Laufer, H.
  15. Comp. Biochem. Physiol v.104B Biochemical characterization for the genetic interpretation of esterase isozymes in Micropogonias furnieri(Pisces, Sciadnidae) in south Brazil Mattiensen, A.;E. Tellechea;J. A. Levy
  16. Biochem. J. v.54 The hydrolysis of esters of phenol by cholinesterases and other esterases Mounter, L. A.;V. P. Whittaker
  17. Comprehensive Insect Physiology, Biochemistry and Pharmacology v.12 Biochemistry and genetics of insecticidal resistance Oppenoorth, F. J.;Kerkut, G. S.;L. G. Gilbert(Eds.)
  18. Appl. Entomol. Zool v.9 Naphthyl acetate esterase in the green rice leafhopper, Nephotettix cincticeps Uhler, with special reference to the resistant colony of the organophosphorus insecticide Ozaki, K.;H. Koike
  19. Insect Biochem v.16 Characterization of cholinesterase activity in larval Chironomus riparius Megen Randall, L. D.;J. C. William
  20. Pesti. Art. News. Summ v.19 Some hydrolase and their involvement in insecticide resistance Sudderuddin, K. I.;H. H. Tan
  21. J. Insect Physiol v.15 Protein changes in the haemolymph of Piers brassicae during the last larval instars and the beginning of the pupal stage Van Der Geest, L. P. S.;F. H. M. Borgsteede
  22. Archs. Insect Biochem. Physiol v.27 Acetylcholinesterase from the horn fly(Diptera: Muscidae) Ⅱ. Biochemical and molecular properties Xu, G.;D. L. Bull
  23. Korean J. Entomol v.23 Isozymatic composition of haemolymph esterase and its ontogeny in Lucilia illustris Yoo, C. M.;S. E. Jeong;H. Y. Kim;H. Y. Ko