한국연초학회지 (Journal of the Korean Society of Tobacco Science)

한국연초학회 (The Korean Society of Tobacco Science)
권호 표지

감자바이러스 Y 복제 유전자로 형질전환된 버어리종 연초의 PVY에 대한 저항성 특성

Resistance to Potato Virus Y Conferred by PVY Replicase Gene Sequence in Transgenic Burley Tobacco

  • Young Ho Kim (Korea Gineng and Tobacco Research Intitute) ;
  • Eun Kyung Park (Korea Ginseng and Tobacco Research Institute) ;
  • Soon Yong Chae (Korea Ginseng and Tobacco Research Institute) ;
  • Sang Seock Kim (Korea Ginseng and Tobacco Research Institute) ;
  • Kyung-Hee Paek (Graduate School of Biotechnology, Korea University) ;
  • Hye Sun Cho (Plant Cell and Molecular Biology Research Unit, KRIBB, KIST)
  • 발행 : 1998.06.01
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초록

The complementary DNA (cDNA) of potato virus Y- vein necrosis strain (PVY-VN) replicase gene (Nlb) was transformed into tobacco (Nicotiana tabacum cv. Burley 21) plants. Out of 25 putative transformants regenerated, 3 were resistant to PVY-VN, one highly resistant plant with no symptom until seed harvest time and the other two with mild chlorotic spot symptoms at late stages after infection. No symptom was observed in the highly resistant plant, while mild vein necrotic symptoms were developed on suckers of the moderately resistant plants after seed harvest time, In the first generation (T1) via self fertilization, resistance to susceptibility frequency in transgenic plants from the highly resistant transformant was about 3 : 1, while it was lowered much (about 1:2 and 1:19) in T1 of the moderately resistant transformants. In the second generation (T2) of the highly resistant plant, resistance frequencies were similar to T1, but resistance levels varied greatly and appeared to be decreased. Key words : potato virus Y, viral replicate gene, transgenic tobacco plants, resistance.

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참고문헌

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