한국양식학회지 (Journal of Aquaculture)

한국양식학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지

LHRH-a 와 pimozide에 의한 동자개의 배란유도

Induction of Ovulation by LHRH-a and Pimozide in the Bullhead, Pseudobagrus fulvidraco

  • 박홍양 (건국대학교 동물자원연구센타) ;
  • 이종영 (건국대학교 동물자원연구센터) ;
  • 이영직 (해양수산부 어업제도과) ;
  • 권혁추 (선문대학교 식량자원학부)
  • 발행 : 1998.05.01
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초록

동자개의 배란유도에 대한 LHRH-a, pimozide 및 HCG의 복강내 투여효과에 대해 조사하였다. 어체중 kg당 HCG를 각각 5,000, 10,0000, 20,000 IU를 주사하였을 경우 71.4, 80, 100%의 배란유도율을 나타냈다. 또한 pimozide의 존재에 관계없이 LHRH-a를 주사한 개체들 대부분이 배란되었다. LHRH-a 단독투여 실험에서 kg당 200, 200, $400^{\mu}$g 처리에서 각각 50, 75 및 100%의 배란유도율을 나타냈다. pimozide첨가는LHRH-a의 작용을 향상시켰다. 즉, LHRH-a와 pimozide의 혼합투여에서 어체중 kg당 pimozide 1 mg과 LHRH-a $300^{\mu}$g에서 100%의 가장 좋은 배란유도율을 나타내었다. 그러나 pimozide 단독투여에서 배란이 유도되지 않았다. 호르몬 처리후의 생식소숙도지수(GSI)와 pesudo-GSI의 변화를 조사한 결과, HCG, LHRH-a 및 LHRH-a+pimozide를 주사한 그룹간의 생식소숙도지수(GSI)와 pseudo-GSI에 있어서 퉁계적인 유의차는 없었다(P>0.05). 호르모처리후 배란이 일어나기까지의 소요시간은 HCG처리후 17~22시간, LHRH-a단독 또는 pimozide와의 혼합처리에서 대체로 23~29시간이 걸렸다.

Effects of intraperitoneal administration of LHRH-a (luteinizing hormone releasing hormone analogue), pimozide and HCG (human chorionic gonadortropin) were studied on ovulation in the bullhead, Pseudobagrus. fulvidraco. Ovulatin was induced in 71.4, 80 and 100% by injection of HCG 5,000, 10,000 and 20,000 IU/kg of body weight, respectively. Also, the majority of fish injected with LHRH-a in the presence or absence of pimozide were ovulated. The ovulating rates in fish injected with LHRH-a alone showed 50, 75 and 100% at a dose 200, 300 and $400^{\mu}$g/kg, respectively. Pimozide enhanced the activity of LHRH-a at $300^{\mu}$g/kg plus pimozide at 1 mg/kg. Pimozide alone was ineffective in inducing ovulation of P. fulvidraco. There were no significant differences in gonadosomatic index (GSI) and pseudo-GSI values among the groups treated with HCG, LHRH-a and LHRH-a plus pimozide (P>0.05). The latency time groups treated with HCG was 17~22 hours, and the group injected with LHRH-a alone and/or in combination with pimozide was ovulated within 23~29 hours.

키워드

참고문헌

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  24. 한국양식학회 추계학술발표대회 요지 LHRH-a 와 pimozide에 의한 한국산 메기의 배란유도 권혁추;김용득;이영직;박흥양
  25. 한국양식학회지 v.9 no.3 생식소자극호르몬 방출호르몬 유사물질에 의한 메기(Silurus asotus)의 배란유도 권혁추;최낙중;박흥양
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