무지개송어(Oncorhynchus mykiss) 배양 간세포에서 Vitellogenin 합성에 미치는 Cu 및 Zn의 억제 효과

Inhibitory Effects of Cu and Zn on Vitellogenin Production in hepatocytes Culture of the Rainbow Trout Oncorhynchus mykiss

  • 발행 : 1998.08.01

초록

Estradiol-17${\beta}$($E_2$)에 의한 Vitellogenin (VTG) 함성에 미치는 Cu 및 Zn의 영향을 무지개송어의 배양 간세포를 이용하여 조사하였다. 간세포는 2일간 배양 한 후, E하(2) ($2{\times}10^6$ M)와 동시에 Cu ($2{\times}10^{-5}$~$10^{-4}$M) 또는 Zn ($10^{-5}$~$10^{-3}$M)을 배양액에 첨가하여 5일간 배양하였다. VTG 합성률은 총 단백질에 대한 VTG의 배율로 나타내었다. CU 및 Zn의 첨가는 배양 간세포의 생존율에는 영향을 미치지 않았다. 그러나, Cu의 첨가에 사용된 모든 농도에서, Zn의 첨가에는 농도 의존적으로 감소하여(10^{-3}$M에서 유의하게 감소하였다. 이러한 감소는, Zn의 제거시에는 회복되었으나, Cu에서는 회복되지 않았다. 그리고, Cu (10^{-4}$M의 첨가 시에 Ca (1.8 mM) 농도를 2.5 및 5.0 mM로 증가시켜도 Cu의 작용을 저해하지 못하였다. 이러한 결과로 보아, Cu 및 Zn은 간세포에서 합성되는 다른 단백질 보다 VTG 합성에 더 깊이 관여하는 것으로 추정된다.

Effects of Cu and Zn on estradiol-17$\beta$-Induced vitellogenin (VTG) production were electro-phoretically examined in hepatocyte cultures of rainbow trout. Hepatocytes were precultured for 2 days and then Cu ($10^{-5}$ ~$10^{-4}$M) and Zn ($10^{-5}$~$10^{-3}$M) were added to the incubation medium with estradiol-17${\beta}$ ($2{\times}10^{-6}$M). The hepatocytes were cultured for 5 more days. The relative VTG production rate was expressed as the percentage of VTG to total proteins including the VTG. The addition of CU and Zn to the incubation medium had no appreciable toxin effect on the viability of hepatocytes in the culture. However, Cu markedly reduced VTG production at any concentration used. Zn also specifically reduced VTG production by hepatocytes in a concentration dependent way and there was a significnt reduction at Zn concentrations of $10^{-3}$M. The reduction recovered by removing Zn from the media, but Cu did not. Additionally, enriched Ca concentrations (1.8 to 2.5 or 5.0 mM) in the incubation medium had no protective effect on the reduction of VTG production by Cu $10^{-4}$ M. These results suggest that the production of VTG is more susceptible to Cu and Zn than are other hepatocyte-derived proteins.

키워드

참고문헌

  1. Chem. Biol. Interact. v.69 Effects of copper on mammalian cell components Agarwal, K.;A Sharma;G. Talukder
  2. Zool. Sci. v.20 Isolation and some chracterization of vitellogenin and its related egg yolk proteins from coho salmon (Oncorhynchus kisutch) Hara, A.;C.V. Sullivian;W.W. dickhoff
  3. Comp. Biochem. Physiol. v.105C Effects of fish concentration of ammonia on cultures hepatocytes of eel (Anguilla japonica) Hayashi, S.;M. Q. Tang;T. Hirakawa;S. Yamada
  4. Can. J. Fish. Aquat. Sci. v.50 Toxicity of trace metal mixtures to alevin rainbow trout (Oncorhynchus mykiss) and larval fathead minnow (Pimephales promelas) in soft, acid water Hickie, B. E.;N. J. Hutchinson;D. E. Dixon;P. V. Hodson
  5. Arch. Environ. Contam. Toxicol. v.18 Lethality of trace metal mixtures to american flagfish in neutralized acid water Hutchinson, N. J.;J. B. Sprague
  6. Comp. Biochem. Physiol. v.104b Vitellogenin induction by estradiol-17β in primary hepatocyte culture in the rainbow trout, Oncorhynchus mykiss Kwon, H.C.;S. Hayashi;Y. Mugiya
  7. Nature v.227 Cleavage of structural proteins during assembly of the head of bacteriophage T₄ Leammli, U. K.
  8. J. Fish. Biol. v.17 Surival and reproductive performance of the desert pupfish, Cyprinodon n. Nevadensis (Eigenmann and Eigenmann), in acid water Lee, R. M.;S. D. Gerking
  9. Gen. Comp. Endocrinol. v.109 Inhibitory effects of aluminium on vitellogenin induction by estradiol-17β in the primary culture of hepatocytes in the rainbow trout Oncorhynchus mykiss Mugiya, Y.;A. Thanahashi
  10. Zoon v.6 Some effects of low pH on the hatchability of eggs of Perch, Perca fluviatilis L. Runn, P.;N. Johansson;G. Milbrink
  11. Toxicol. Appl. Pharmacol. v.110 Interaction of copper with DNA and antagonism by other metals Sagripanti, J. L.;P. L. Goering;A. Lamanna
  12. J. Fish Biol. v.35 The effect of calcium concentration on the toxicity of copper, lead and zinc to yolk-sac fry of brown trout, Salmo trutta L., in soft, acid water Sayer, M. D. J.;J. P. Reader;R. Morris
  13. Com. Biochem. Physiol. v.C102 The influence of calcium and humic substances on aluminium accumulation and toxicity in the minnow, Phoxinus phoxinus, at low pH Wicklund-Glynn, A.;L. Norrgren;O. Malmborg
  14. Zn 亞鉛 Toxicology Today 小山 洋;佐藤 洋(編)
  15. Cu. 銅 Toxicology Today 遠山 千春;本間 志乃;佐藤 洋(編)