한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제27권2호
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  • Pages.319-325
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  • 1998
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지

Streptozotocin 유발 당뇨쥐의 신장 및 뇌조직에서의 Microsomal Mixed Function Oxidase System에 미치는 녹차 Catechin의 영향

Effect of Green Tea Catechin on the Microsomal Mixed Function Oxidase System of Kidney and Brain in Streptozotocin-Induced Diabetic Rats

  • 이순재 (대구효성가톨릭대학교 식품영양학과) ;
  • 신주영 (대구효성가톨릭대학교 식품영양학과) ;
  • 차복경 (대구효성가톨릭대학교 식품영양학과)
  • 발행 : 1998.04.01
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초록

The purpose of this study was to investigate the effect of green tea catechin on microsomal mixed function oxidase(MFO) system of kidney and brain in streptozotocin(STZ) induced diabetic rats. Sprague-Dawley male rats weighing 140$\pm$10g were randomly assigned to one control and three STZ-diabetic groups. Diabetic groups wer classified to DM-0C(catechin 0%/kg diet), DM-0.5C (catechin 0.5%/kg diet), and DM-1.0C(catechin 1%/kg diet) according to the level of catechin supplementation. Diabetes were experimentally induced by intravenous administration of 55mg/kg body weight of STZ in citrate buffer(pH 4.3) after 4 weeks feeding of three experimental diets. Animals were sacrificed at the sixth day of diabetic state. The contents of cytochrome P450 in kidney were increased by 77, 42, 49% in DM-0C, DM-0.5C and DM-1.0C groups, respectively, than normal group. The contents of cytochrome P450 in brain were increased by 43% in DM-0C group than normal group, but those of DM-0.5C and DM-1.0C groups were similar to that of normal group. The contents of cytochrome b5 in kidney were increased by 78, 38, 49% in DM-0C, DM-0.5C and DM-1.0C groups, respectively, than normal group. Meanwhile, the contents of cytochrome b5 in brain were not significantly different among all groups. The activities of NADPH-cytochrome P450 reductase in kidney of DM-group were increased by 27% than normal group, but those of DM-0.5C and DM-1.0C groups were 13 and 15% lower than that of DM-0C group. The activities in brain were also increased by 31% in DM-0C group, but those of DM-0.5C and DM-1.0C groups were similar to than of normal group. Levels of TBARS (thiobarbituric acid reactive substance) in kidney were increased by 147, 60 and 59% in DM-0C, DM-0.5C, and DM-1.0C groups, respectively, compared with normal group, but those of DM-0.5C and DM-1.0C groups were 36, 35% lower than that of DM-0C group. Meanwhile, the levels of TBARS in brain were not significantly different among four groups. These results indicate that dietary catechins in green tea play a powerful antioxidant role in reducing the lipid peroxidation enhanced by activation of MFO system in STZ-induced diabetes.

키워드

참고문헌

  1. Sci. v.201 The biology of oxygen radicals, the superoxide radical as an agent of oxygen toxicity: superoxide dismutase provides an important defense Frielovich, I.
  2. J. Biol. Chem. v.224 Superoxide dismutase: an enzymatic function for erythrocupreine(hemocupreine) McCord, J. M.;Fridovich, K.
  3. Diabetes v.35 Triglyceride-lowering effects of dietary vitamin E in streptozotocin induced diabetic rats. Increased lipoprotein lipase activity in livers of diabetic rats fed high vitamin E Prichard, K. A.;patel, S. T.;Karper, C. W.;Newman, H. A. I.;panganamala, R. V.
  4. Diabetes v.34 Production of 12-hydroxyeicosatetraenoic and vitamin E status in platelets from type Ⅰ human diabetic subjects Karpen, C. W.;Cataland, S.;O'Doriso, T. M.;Panganamala, R. V.
  5. Am. J. Med. v.49 the role of the liver in durg metabolism Remmer, H.
  6. Cancer Res. v.30 Carcinogenesis by chemicals; an overview - GHA Clowes memorial leuture Miller, J. A.
  7. Biochem. Biophys. Acta v.673 Characterization of the induction of drug-metabolizing enzymes by 2-acetylaminofluorene Astrom, A.;Depierre, J. W.
  8. Lipids v.8 Damage to microsomal membrane by lipid peroxidation Bidlack, W. T.;Tappel, A. L.
  9. 한국영양학회지 v.23 2-Acetylaminofluorene과 choline 결핍이 서로 다른 지방을 섭취한 쥐간의 지질과산화 반응 및 glucose 6-phosphatase, glutathione S-transferase 활성도에 미치는 영향 김현아;최혜미
  10. 한국식품영양과학회지 v.25 식이 vitamin E가 streptozotocin 유발 당뇨쥐의 간 및 폐조직에서의 microsomal mixed function oxidase system에 미치는 영향 박영란;이순재;임영숙;주길재
  11. J. Nutr. v.112 Effects of dietary selenium and vitamin E on hepatic mixed function oxidase activities and in vivo covalent binding of aflatoxin B₁in rats Chen, J.;Mabel, P.;Goetchirs, T.;Campbell, C.;Getald, F. C. JR.
  12. Food Chem. v.20 Vitamin E regulation of the biosynthesis of porphyrins and heme Nair, P. P.;Murty, H. S.;Cassi, P. I.;Brooks, S. K.;Quartner, J.
  13. 한국영양식량학회지 v.23 흰쥐 간에 있어 지질과산화에 미치는 식이성 비타민 E의 수준 및 카페인의 영향 박미리;조수열
  14. J. Nutr. Sci. Vitaminol. v.32 Effect of tea catechins on plasma cholesterol fed rats Muramatsu, K.;Fukuyo, M.;Hara, Y.
  15. Kansenshogaku Zasshi v.68 Effect of catechin on the ultrastructure of Trichophyton mentagraphytes Toyoshima, Y.;Okubo, S.;Toda, M.;Shimamura, T.
  16. 浦上財團硏究報告書 v.1 綠茶生藥の藥理效果について 林榮一
  17. Japan. J. Cancer Res. v.80 Inhibitory effect of (-)-epigallocatechin gallate on carcinogenesis with N-ethyl-N'-nitro-N-nitrosoguanidine in mouse duodenum Fujita, Y.;Yamane, T.;Tanka M.;Kuwata, K.;Okuzumi, J.;Takehashi, T.;Fujiki, H.;Okuda, T.
  18. Oyo. Yakuri v.40 Pharmacological cation of tea extract on the central nervous system in mice Hayashi, E.;Hayashi, M.;Yamazoe, H.
  19. 한국영양식량학회지 v.23 한국산 녹차, 우롱차 및 홍차 음료의 cadmium 제거작용에 관한 연구 김미지;이순재
  20. 한국영양학회지 v.25 녹차 추출물의 항산화 효과 이주원;신효선
  21. 茶葉硏究報告 v.71 茶の分析法 池ケ俗 賢次部;高柳博次;何南農正
  22. J. Biol. Chem. v.239 The carbon monoxide-binding pigment of liver microsomes Omura, T.;Sato, R.
  23. Meth. Enzymol. v.10 The preparation and properties of microsomal TPNH-cytochrome C reductase from pig liver Master, B. S. S.;Williams, C. H.;;Karmin, H.
  24. Clinica. Chem. Acta v.90 Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method Satoh, K.
  25. J. Boil. Chem. v.193 Protein measurement with the folin phenol reagent Lowry, O. H.;Rosenbrouth, N. J.;Farr, A. L.;Randll, R. J.
  26. SPSS/PC를 이용한 통계 분석 채서일;김범룡
  27. Biochem. Biophys. Acta v.673 Characterization of the induction drug-metabolizing enzymes by 2-acetylaminofluorene Astrom, A.;Di Pierre, J. W.
  28. 한국생화학회지 v.27 급성 납중독된 흰쥐의 이물질 대사효소의 활성도에 관한 연구 김기웅;송용범
  29. J. Biol. Chem. v.261 Preparation and characterization of FAD-dependent NADPH-cytochrome $P_{450}$ reductase Gary, P. K.;Henry, W. S.
  30. Cancer Res. v.44 Changes in molecular forms of rat hepatic glutathione S-transferase during chemical hepato-carcinogenesis Kitahara, A.;Satoh, K.;Nishimura, K.;Ishikawa, T.;Ruike, K.;Sato, K.;Tsuda, H.;Ito, N.
  31. Ann. NY. Acad. Sci. v.393 The effect of vitamin E on arachidonic acid metabolism Panganamala, R. V.;Corn, D. G.
  32. Res. Common. Chem. Pathol. Pharmacol. v.28 Vitamin E and inhibition of patelet lipoxygenase Gwebu, E. T.;Trwyn, R. W.;Cornwell, D. G.;Panganamala, R. V.
  33. 한국영양식량학회지 v.24 Streptozotocin 유발 당뇨쥐에 있어서 metallothionein 합성에 미치는 비타민 E의 영향 최원경;이순재