The Korean Journal of Food And Nutrition (한국식품영양학회지)
- Volume 11 Issue 4
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- Pages.381-387
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- 1998
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- 1225-4339(pISSN)
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- 2287-4992(eISSN)
Production of Streptomyces albus KSM-35 Amylase from Bacillus subtilis LKS88 Haboring the Recombinant Plasmid pASA240
재조합 균주 Bacillus subtilis LKS88에 의한 Streptomyces albus KSM-35 Amylase의 생산조건
Abstract
The effects of culture conditions on the production of amylase expressed by Bacillus subtilis LKS88 with a cloned gene from Streptomyces albus KSM-35 were investigated. The production of amylase was increased significantly by using sodium citrate and rice hull as a carbon source. In addition, the use of a mixture of sodium citrate and rice hull (1:1) resulted in increase of enzyme production by 20-fold when compared to that of soluble starch. The soybean meal as the nitrogen source could be partially replaced with yeast extract without changing the enzyme production yield. The amylase production was also increased by adjusting initial pH to 6.0 or by adding 0.01% SDS. Maximum amylase production was observed in the medium containing 1.5% sodium cirtate, 1.5% rice hull, 0.7% soybean meal, 0.3% yeast extract, 0.66% K2HPO4, 0.05% MgSO4