Preventive Nutrition and Food Science
- Volume 3 Issue 4
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- Pages.315-319
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- 1998
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- 2287-1098(pISSN)
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- 2287-8602(eISSN)
Lipoxygenase Inhibition and Antioxidative Activity of Flavonoids from Paeonia moutan Seeds
- Kim, Hyo-Jin (Dept.of Food Science and Nutrition, Catholic University of Taegu-Hyosung) ;
- Chung, Shin-Kyo (Dept. of Food Science and Technology, kyungpook National University) ;
- Park, Sang-won (Dept. of Food Science and Nutrition, Catholic University of Taegu-Hyosung)
- Published : 1998.12.01
Abstract
Previously, the methanolic extract of Paeonia moutan seeds was found to potently inhibit soybean lipoxy-genase (SLO). Hence to isolate SLO inhibitor, the defattd methaniolic extract of the seeds was consecutively partitioned wiht ether, ethyl acetate,n-butanol ,adn water. The ether souble fraction showing strong inhibitory activity against SLO was further fractionated into a strongly acidic, a weakly acidic, and a neutral fractions. The strongly acidic components of the ether extract were successively subjected to chromatography on a silica gel, Sephadex LH-20, and preparative HPLC. Four phenolic compounds were isolated , and twio of them showing a strong SLO inhibition activity were identified as luteolin (IC50=2.32
Keywords
- Paeonia moutan seeds;
- soybean lipoxygenase inhibitor;
- antioxidants;
- luteolin and 5,6,4′-trihydroxy-7, 3′-dimethoxyflavone