버섯에서 분리한 형광성 Pseudomonas spp. 의 ITS I 영역 분석에 의한 계통 분류

Phylogeneitc Analysis of Fluorescent Pseudomonas spp. Isolated from the Cultivated Mushrooms on the Basis of ITS I Region

  • 고승주 (농업과학기술원 분자유전과) ;
  • 고승주 (농업과학기술원 분자유전과) ;
  • 강희완 (농업과학기술원 세포유전과) ;
  • 전명숙 (농업과학기술원 분자유전과) ;
  • 류진창 (농업과학기술원 분자유전과)
  • 발행 : 1998.08.01

초록

A total of 12 strains of fluorescent Pseudomonas isolated from the cultivated mushrooms such as Agaricus bisporus and Pleurotus ostreatus were collected. They consisted of pathogenic Pseudomonas spp. and epiphytic Pseudomonas spp. of the cultivated mushroom. To analyze the phylogenetic relationship of these strains, ITS I region, the 16S-23S intergenic spacer region in the ribosomal RNA (rRNA) operon, was cloned and sequenced. The spacer regions of these strains were 495∼527 nucleotides in length and contained the genes encoding isoleucine-tRNA (tRNAIle) and alanine-tRNA (tRNAAla). The reciprocal homologies of each ITS I sequence among these strains were in the range of 84.2%∼98.8%. According to the analysis of ITS I sequences, the fluorescent Pseudomonas spp. were phylogenetically classified into three clusters. Cluster I consisted of Pseudomonas fluorescens, P. tolaasii, P. gingeri’, and P.‘reactans’(WLRO). Cluster II comprised Pseudomonas fluorescens biovar C and F. Cluster III composed P. agarici. Cluster I and II could be classified into P. fluorescens complex. P. agarici formed an independent taxon clearly separable from P. florescens complex.

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