감귤 잿빛곰팡이병균의 살균제에 대한 저항성 및 유전적 다양성

Fungicide Resistance and Genetic Diversity of Botrytis cinerea of Citrus

  • 고영진 (순천대학교 응용생물원예학부) ;
  • 이재군 (순천대학교 응용생물원예학부) ;
  • 서정규 (순천대학교 응용생물원예학부) ;
  • 문두길 (제주대학교 원예생명과학부) ;
  • 한해룡 (제주대학교 원예생명과학부)
  • 발행 : 1998.12.01

초록

Fungicide resistance of 48 isolates of Botrytis cinerea collected from citrus in Cheju was investigated and genetic diversity was analyzed with random amplified polymorphic DNA(RAPD). High levels of resistance to benzimidazole fungicides benomyl and thiophanate-methyl and N-phenylcarbamate fungicide diethofencarb were observed. Negative cross resistance was clear between benzimidazole and N-phenylcarbamate fungicides, and multiple resistance to the fungicides was also observed. There was cross resistance among the dicarboximide fungicides procymidione, vinclozolin and iprodione as it was observed between the benzimidazole fungicides benomyl and thiophanate-methyl. The lowest levels of resistance were to the dicarboximide fungicides, but no sensitive isolate to polyoxin B was observed. The isolates showed genetically diverse RAPD profiles according to the geographic origin collected, but there was no significant correaltion between RAPD profiles of genomic DNA and the levels of fungicide resistance of the isolates. The isolates showed genetically diverse RAPD profiles, indicating that genetic differentiation had already occurred in the populations of B. cinerea distributed in Cheju.

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