Journal of Embryo Transfer (한국수정란이식학회지)
- Volume 13 Issue 1
- /
- Pages.1-9
- /
- 1998
- /
- 2508-755X(pISSN)
- /
- 2288-0178(eISSN)
Post-thaw Embryo Development following Vitrification or Slow Freezing of Rabbit Normal and Nuclear Transplant Embryos
토끼의 정상 및 핵이식배의 유리화 및 완만동결에 따른 융해 후 발달율
Abstract
In order to improve the cryopreservation by vitrification or slow freezing of nuclear transplant rabbit embryos, the effects of factors affecting embryo cryopreservation such as cryoprotectants, equilibration, cooling rate and post-thaw dilution on post-thaw survial and development were determined using intact embryos of morular stage. And the post-thaw development of nuclear transplanted embryos cryopreserved under the optimal conditions examined was compared between vitrification and slow freezing. The cryoprotectant solution used was ethyleneglycol-ficoll-sucrose (EFS) or ethyleneglycol-poly-vinylpyrrolidone-galactose- I (EPG- I ) for vitrification, and EPG- II for slow freezing. To examine the viability of frozen-thawed embryos, the nuclear transplanted embryos were co-cultured in TCM-199 plus 10% FBS with bovine oviduct epithelial cells(BOEC) for 24 hrs and the intact morulae were co-cultured with BOEC for 5 days and 3 days to hatching blastocyst stage in 39 ˚C 5%