KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Influence of Amino Acid and Vitamin Addition on the Growth and Metabolism of a Hybridoma in Batch Culture

하이브리도마의 회분식배양에서 아미노산과 비타민의 첨가에 따른 세포성장과 대사의 변화

  • 이동섭 (한양대학교 화학공학과) ;
  • 박홍우 (한양대학교 화학공학과)
  • Published : 1998.06.01
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Abstract

The effects of various step-fortifications of the initial medium with amino acids, glucose, and vitamines on the growth and metabolism of a hybridoma cell line in batch cultures were quantified. Comparisons between the metabolic rates of the various cultivations were made for the exponential growth phase. Fortification of the basal medium resulted in higher cell densities through a prolonged growth phase, but the maximum specific growth rate was not affected. The uptake rate of glutamine increased with the addition of amino acids but did not change upon the addition of glucose or vitamines. The specific glucose consumption decreased slightly with the addition of amino acids but increased production of lactate and {{{{ { NH}`_{4 } ^{ +} }}}}. A reciprocal relationship between the yields of {{{{ { NH}`_{4 } ^{+ } }}}} and lactate indicated a joint regulation of glycolysis and glutaminolysis.

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References

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