KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Bioreactor Operating Strategy in Scultellaria baicalensis G. Plant Cell Culture for the Production of Flavone Glycosides

Flavonoid 배당체 생산을 위한 Scutellaria baicalensis G. 식물 세포 배양에서 생물반응기 운전전략

  • 최정우 (서강대학교 공과대학 화학공학과) ;
  • 조진만 (서강대학교 공과대학 화학공학과) ;
  • 이정건 (서강대학교 공과대학 화학공학과) ;
  • 이원홍 (서강대학교 공과대학 화학공학과) ;
  • 김익환 (고려대학교 생명공학원) ;
  • 박영훈 (한국과학기술원 생명 공학 연구소 생물 자원 공정 연구부)
  • Published : 1998.06.01
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Abstract

Optimal feeding strategies in bioreactor operation of Scutellaria baicalensis G. plant cell culture were investigated to maximize the production of flavone glycosides by using a structured kinetic model which can predict culture growth and flavone glycosides synthesis in a rigorous, quantitative manner. For the production of baicalin and wogonin-7-0-GA, the strategies for glucose feeding into Scutellaria baicalensis G. plant cell culture were proposed based on the model, which are a periodic fed-batch operation with maintenance of cell viability and of specific production rate respectively, and a perfusion operation with maintenance of specific production rate for baicalin and wogonin-7-0-GA. Simulation results showed that the highest volumetric concentration of flavone glycosides was obtained in a periodic fed-batch operation with maintenance of cell viability among all the suggested strategies. In the periodic fed-batch operations, the higher volumetric production of flavone glycosides was achieved compared with that in the perfusion operation. It can be concluded that a periodic fed-batch operation with maintenance of cell viability would be the optimal and practical operating strategy of Scutellaris baicalensis G. plant cell culture for the production of flavone glycosides.

Keywords

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