KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Induction and Culture of Hairy Roots of Crotalaria sessiliflora L.

활나물(Crotalaria sessiliflora L.)로부터 모상근의 유도 및 배양

  • Kim, Young-Jun (Department of Biology, Chonnam National University) ;
  • Pyo, Byoung-Sik (Department of Food and Biotechnology, Dongshin University) ;
  • Kim, Kwang-Soo (Department of Biology, Chonnam National University) ;
  • Hwang, Baik (Department of Biology, Chonnam National University)
  • 김영준 (전남대학교 생물학과) ;
  • 표병식 (동신대학교 식품생물공학과) ;
  • 김광수 (전남대학교 생물학과) ;
  • 황백 (전남대학교 생물학과)
  • Published : 1998.04.01
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Abstract

The hairy roots of Crotalaria sessiliflora were induced from the tissue segments infected with Agrobacterium rhizogenes ATCC 15834. The induced hairy roots were subjected to paper electrophoresis fro the detection of opine-positive clones which were considered to have been transformed. Mannopine and agropine were presented in hairy root clones while mannopine was presented in two hairy root clones. Eight hairy root clones were selected and cultured in MS, B5 and WP media. Each of hairy root clones was showed a difference in branch pattern and growth rate. The best culture medium and culture conditions of hairy roots were in $\frac{1}{2}$MS(3% sucrose, pH 5.7) liquid medium at 25$^\circ C$, 70 rpm under dark, the growth rate in $\frac{1}{2}$MS liquid medium was increased with 210-fold more than that of inoculated hairy roots and with 2-fold more than that in MS liquid medium. Also, the adequate condition for hairy root growth was such that concentration of KH$_2PO$_4 was 1.25mM and the ratio of NH${_4}{^+}$ : NO${_3}{^-}$ was 1 to 3 in MS medium. The presence of pyrrolizidine alkaloids, monocrotaline, in the hairy roots was detected by TLC.

Keywords

References

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