Phenol 분해 균주의 분리 및 특성

Isolation and Characterization of a Phenol-Degrading Bacteria

  • 발행 : 1998.04.01

초록

Twelve bacterial strains capable of growing on phenol minimal medium were isolated from iron foundry activated sludge by enrichment culture, and amount them, one isolate which was the best in cell growth and phenol degradation was selected and identified as Acinetobacter junii POH. The optimal temperature, initial pH and phenol concentration in the above medium were 3$0^{\circ}C$, 7.5 and 1000 ppm, respectively. Cell growth of Acinetobacter junii POH dramatically increased 20 hrs cultivation-time and reached a almost stationary phsae 40 hrs cultivation-time then phenol was degraded about 98%. Cell growth was inhibited y phenol at concentrations over 1500 ppm. The isolate was resistant to several antibiotics as well as various heavy metal ions. The growth-limiting log P value of Acinetobacter junii POH on organic solvents was 2.9 in the LB medium. Therefore, it is suggested that Acinetobacter junii POH could be effectively used for the biological treatment of wastewater containing the presence of heavy metal ions and organic solvents.

키워드

참고문헌

  1. Biotechnol. Bioeng. v.41 Phenol Removal from Waste Gases with a Biological Filter by Pseudomonas putida Zilli,M.;A.Converti;A.Lodi;M.Del Borghi;G.Ferraiolo
  2. Biotechnol. Lett. v.9 Selection and Adaptation of a Phenol-Degrading Strain of Pseudomonas Masque,C.;M.Nolla;A.Brodons
  3. Environ. Sci. Technol. v.16 Microbial Removal of Hazardous Organic Compounds Kobayashi,H.;B.E.Rittman
  4. Environ. Prog. v.2 Industrial Wastewater Treatment with a New Biological Fixed-Film System Stensel,H.D.;S.Reiber
  5. Biotechnol. Lett. v.11 Kinetics and Fluorometric Behaviour of a Phenol Fermentation Li,J.K.;A.E.Humphrey
  6. Biotechnol. Bioeng. v.37 The Biodegradation of Mixtures of Organic Solvents by Mixed and Monocultures of Bacteria Bitzi,U.;T.Egli;G.Hammer
  7. Appl. Microbiol. Biotechnol. v.39 Degradation of Polycyclic Aromati Hydrocarbons by an Immobilized Mixed Bacteria Culture Weisel,I.;S.M.Wubker;H.J.Rehm
  8. Appl. Microbiol. Biotechnol. v.30 Degradation of Phenol by a Coimmobilized Entrapped Mixed Culture Zaxhe,G.;H.J.Rehm
  9. Kor. J. Appl. Microbiol. Biotechnol. v.22 Isolation and Characterization of a Phenol-Degrading Strain Acineto-bacter sp. GEM2 Lee,C.H.;H.M.Oh;T.J.Kwon;G.S.Kwon;S.G.Lee;H.H.Suh;B.D.Yoon
  10. Kor. J. Appl. Microbiol. Biotechnol. v.23 Characterization of Trichloroethylene and Phenol Degradation by Acinetobacter sp. T5-7 Hong,S.Y.;S.H.Lee;J.H.Lee;J.H.Ha
  11. Biotechnol. Bioeng. v.41 Kinetics of Competitive Inhibition and Competabolism in the Biodegradation of Benzene, Toluene and p-Xylene by Two Pseudomonas Isolates Chang,M.K.;T.C.Voice;C.S.Criddle
  12. Appl. Microbiol. Biotechnol. v.37 Degradation of Phenol and Phenolic Compounds by Pseudomonas putida EKⅡ Hinteregger,C.R.;M.Leitner;J.Loidl.;A.Fersch;F.Streichsbier
  13. Appl. Microbiol. Biotechnol. v.26 Degradation of Phenol by a Mixed Culture of Pseudomonas putida and Cryptococuus elinovii Adsorbed on Activated Carbon Morsen,A.;H.J.Rehm
  14. Appl. Microbiol. Biotechnol. v.30 Semicontinuous and Continuous Degradation of Phenol by Pseudomonas putida P8 Adsorbed on Activated Carbon Ehrhardt,H.M.;H.J.Rehm
  15. Kor. J. Appl. Microbiol. Biotechnol. v.16 Isolation and Characterization of Pseudomonas putida N3 Degrading Naphthalene Kho,Y.H.;I.H.Ha;K.S.Bae
  16. Bergey's Manual of Determinative Bacteriology(9rd ed.) Holt,J.G.;N.R.Krich;P.H.Sneath;J.T.Staley;S.T.Williams
  17. The Prokaryotes(2nd ed.) v.Ⅳ BaLows,A.;H.G.Truper;M.Dworkin;W.Harder;K.H.Schleifer
  18. Biochemical Test of Identification of Medical Bacteria Macfaddin,J.F.
  19. J. Gen Appl. Microbid. v.29 The Coenzyme Q System in Strains of Some Yeasts and Yeast-Like Fungi Yamada,Y.I.;Ohishi;K.Kondo
  20. FEMS Microbiol. Lett. v.25 Determination of DNA Base Composition by Reversed-Phase HPLC Tamaoka,J.;K.Komugata
  21. Appl. Environ. Microbiol. v.58 Physical Properties of a Pseudomonas Strain Which Grows with p-Xylene in a Two-Phase(Organic-Aqueous) Medium Diana,L.C.;J.H.Wolfman;R.D.Rogers;D.T.Gibson
  22. J. Ferment. Bioeng. v.71 Estimation of Solvent-Tolerance of Bacteria by the Solvent Parameter Log P Inoue,A.;K.Horikoshi
  23. Appl. Environ. Microbiol. v.56 Phenol and Trichloroethylene degradation by Pseudomonas cepacia G4 : Kinetics and Interactions between Substrates Folsom,B.R.;P.J.Chapman;P.H.Pritchard