셀룰라아제와 베타글루코시다아제의 혼합효소를 사용한 섬유소-가수분해반응의 최적조건

Optimum Conditions of Cellulose-Hydrolysis Reaction with Mixed Enzymes of Cellulase and $\beta$-Glucosidase

  • 손민일 (명지대학교 공과대학 화학공학과) ;
  • 김태옥 (명지대학교 공과대학 화학공학과)
  • 발행 : 1998.02.01

초록

Optimum conditions of the cellulose-hydrolysis reaction with mixed enzymes(cellulase extracted from Penicellium funiculosum mixed with $\beta$-glucosidase extracted from Almod) were investigated to increase the production of glucose from cellulose. Experimental result showed that optimum conditions fro pH, activity ratio of $\beta$-glucosidase to cellulase, concentration of mixed enzymes, concentration of cellulose as a substrate, and temperature range were 4.2, 0.4, 0.8, U/mL, 40 g/L, and 37$\pm$3$^\circ C$, respectively. In these conditions, quantities of glucose productions by using mixed enzymes were larger than those by using cellulase at optimum conditions.

키워드

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