Ammonia Production from Yeast Extract and Its Effect on Growth of the Hyperthermophilic Archaeon Sulfolobus solfataricus

  • Park, Chan-Beum (Department of Chemical Engineering, Pohang University of Science and Technology) ;
  • Lee, Sun-Bok (Department of Chemical Engineering, Pohang University of Science and Technology)
  • Published : 1998.12.01

Abstract

Utilization of yeast extract and formation of byproduct metabolite were investigated for hyperthermophilic archaeon Sulfolobus solfataricus (DSH 1617). In both batch and fed-batch cultivations of S. solfataricus, maximal cell density, {{{{ { NH}`_{4 } ^{ +} }}} ion production and pH change were highly dependent on the ratio of yeast extract to glucose in the medium. Variation of {{{{ { NH}`_{4 } ^{ +} }}}} ion level was identified as a major cause of pH change during cultivation, and acidification of culture broth was attributed to consumption of {{{{ { NH}`_{4 } ^{ +} }}}} ions rather than formation of acid byproducts. It was also observed that increase of {{{{ { NH}`_{4 } ^{ +} }}}} ion concentrations in the medium resulted in greater degree of growth inhibition.

Keywords

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