Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Purification and Structural Characterization of Glycolipid Biosurfactants from Pseudomonas aeruginoas YPJ-80

  • Park, Oh-Jin (BioProcess Engineering Research Center, Department of Chemical Engineering, Korea Advanced Institute of Science and Technology Taejon) ;
  • Lee, Young-Eun (BioProcess Engineering Research Center, Department of Chemical Engineering, Korea Advanced Institute of Science and Technology Taejon) ;
  • Cho, Joong-Hoon (BioProcess Engineering Research Center, Department of Chemical Engineering, Korea Advanced Institute of Science and Technology Taejon) ;
  • Shin, Hyun-Jae (BioProcess Engineering Research Center, Department of Chemical Engineering, Korea Advanced Institute of Science and Technology Taejon) ;
  • Yoon, Byung-Dae (Environmental Microbiology Research Unit, Korea Research Institute of Bioscience and Biotechnology, KIST, Taejon) ;
  • Yang, Ji-Won (BioProcess Engineering Research Center, Department of Chemical Engineering, Korea Advanced Institute of Science and Technology Taejon)
  • Published : 1998.12.01
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Abstract

Glycolipids produced by Pseudomonas aeruginosa YPJ-80 were characterized by chromatographic and spectorscopic techniques as a mixture of two rhamnolipids. For recovery of glycolipids from the culture broth, various isolation methods including ultrafiltration, adsorption and solvent extraction were compared. Ultrafiltration showed the best results in terms of glycolipids recovery. Further purification for spectroscopic analysis was carried out by adsorption chromatography and preparative thin layer chromatography. From the spectroscopic analysis, such as IR spectroscopy. FAB-MS, 1H-NMR and 13C-NMR and hydrolysis analysis, the glycolipids were identified as L-${\alpha}$-rhamnopyranosyl-${\beta}$-hydroxydecanoly-${\beta}$-hydroxydecanoate and 2-O-${\alpha}$-L-rhamnopyranosyl-${\alpha}$-L-rhamnopyranosyl-${\beta}$-hydroxydecanoyl-${\beta}$-hydroxydecanoate. Monorhamnolipid and dirhamnolipid lowered the surface tension of water to 28.1 mN/M and 29.3 mN/m, respectively.

Keywords

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