Thermal Stabilization of Aspergillus phytase by L-Arginine

  • Sunghoon Ryu (Department of Biotechnology, Chung Ang University) ;
  • Park, Tae-Gwan (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
  • 발행 : 1998.06.01

초록

Phytase from Aspergillus species is a very heat unstable enzyme which inactivates to a great extent during the thermal processing of animal feed formulation. Various protein stabilization additives were tested to improve its heat stability. Among them, a basic amino acid, L-arginine remarkably increased the thermal stability of phytase in an aqueous solution state.

키워드

참고문헌

  1. J. Agric. Food Chem v.42 Substrate selectivity in Aspergillus ficuum phytase and acid phosphatases using myo-inositol phosphates Ullah, A. H.;B. Q. Phillipy
  2. Chemistry and Application of Phytic acid: an overview Graf, E.
  3. J. Agr. Sci v.62 Microbial phytase supplementation for improving availability of plants phosphorous in the diet of growing pigs Nasi, M.
  4. Bio/technology v.11 Phytase-containing transgenic seeds as a novel feed additive for improved phosphorous utilization Pen, J.;T. C. Verwoerd;A. Hoekema
  5. Pharm. Res v.6 Stability of protein pharmaeuticals Manning, M. C.;K. Patel;R. T. Borchadt
  6. Stability of Protein Phamaceuticals. PartA: Chemical and Physical Pathways of Protein Degradation Volkin, D. B.;C. R. Middaugh;Ahern, T. J.(ed.);Manning, M. C.(ed.)
  7. Anal. Biochem v.113 A new and convenient colorimetric determination of inorgani orthophophates and its application to the assay of inorganic pyrophosphatase Heinonen, J. K.;R. J. Lahiti
  8. Stability and Characterization of Protein and Peptide Drugs: Case Histories Nguyen, T. H.;C. Ward;Wang, Y. J(ed.);Pearlman, R.(ed.)