Biotechnology and Bioprocess Engineering:BBE

  • 제3권1호
  • /
  • Pages.19-23
  • /
  • 1998
  • /
  • 1226-8372(pISSN)
  • /
  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Sensitivity Enhancement of Surface Plasmon Resonance Biosensor with Colloidal Gold

  • Kibong Choi (GWSD-4-3, Agency for Defense Development) ;
  • Hee (GWSD-4-3, Agency for Defense Development GWSD-4-3, Agency for Defense Development Department of Biochemistry, Chungbuk National University)
  • 발행 : 1998.06.01
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초록

We enhanced the sensitivity of surface plasmon resonance biosensor by the conversion of the real-time direct binding immunoassay into the sandwich immunoassay, in which colloidal gold particles coated with anti-mouse IgG was used. By the immobilization of anti-mouse IgG onto the carboxymethyl dextran surface of thin gold film, the direct binding of analyte(mouse IgG) onto the sensor chip, and the injection of colloidal gold particles coated with anti-mouse IgG, about 100 times of sensitivity enhancement was obtained. This result suggests that nanoparticles, which has a high refractive index, homogeneous ultrafine structure and capability of size control, would be applicable for the detection of very small quantity of biomaterial.

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참고문헌

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