생명과학회지 (Journal of Life Science)

  • 제8권4호
  • /
  • Pages.426-430
  • /
  • 1998
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

RAPD 마커를 이용한 멧누에와 집누에 계통간의 분자적 유연관계 분석

Analysis of Molecular Relationships Between Bombyx mandarina and Bombyx mori Strains Using RAPD-Markers

  • 황재삼 (농촌진흥청 잠사곤충연구소) ;
  • 이진성 (생명공학연구소 유전체사업단) ;
  • 구태원 (농촌진흥청 잠사곤충연구소) ;
  • 강현아 (농촌진흥청 잠사곤충연구소) ;
  • 손해룡 (경복대학교 농과대학) ;
  • 김호락 (농촌진흥청 잠사곤충연구소)
  • 발행 : 1998.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 RAPD마커를 이용, 멧누에와 집누에의 분자적 유연관계를 분석하였다. 공시한 35개의 primer에서 166개의 RAPD마커를 얻었으며, 이들 마커를 UPGMA에 의해 분석한 결과, 멧누에와 분자적 유사계수가 가장 낮은 품종은 잠305였고, 가장 높은 품종은 Bibaekjam이었다. 또한, 분자적 유사계수 0.55에서 멧누에와 집누에 계통군으로 분류되고, 0.60에서 3개의 아군 그룹과 2개의 독립개체로 분류되었다. 제1아군에는 J111(일본종계),$pnd^{ps}$(일본종계), Bibaekjam(일본종계)이, 제2아군에는 Galwon(중국종계), C18(중국종계), od yujam JAM306(중국종계), C108(중국종계)이, 제3아군에는 R-hwang(중국종계)이 포함되어 있었고, zebra(유럽종계)와 JAM305(일본종게)는 독립개체로 분류되었다.

The molecular relationships have analyzed between the Bombyx mandarina(wild silkworm) and Bombyx mori strains (domesticated silkworm, geographical silkworms). A total of 166 polymorphic RAPD markers amplified from 35 different primers were used to analyze the molecular relationships among thirteen silkworm strains. The genetic similarity coefficient between Bombyx mandarina and Jam305 showed the lowest genetic similarity value with 0.451, Bombyx mandarina and Bibaekjam showed the highest genetic similarity value with 0.958. These strains were classified into Bombyx mandarina(a wild silkworm) and Bombyx mori(twelve domesticated silkworm) groups upon the genetic similary coefficient of 0.55. Further classificient of 0.60; the 1st sub-group (J111, Bibaekjam, $pnd^{ps}$), the 2nd sub-group (Galwon, C18, od yujam, JAM306, C108), the 3rd sub-group(R-hwang, p50), the 4th sub-group(zebra) and the 5th sub-group(JAM305). According to this study, RAPD markers seems to be a valuable tool for molecular relationships and classification among the silkworms.

키워드

참고문헌

  1. Jpn. J. Sericult. Sci. v.64 no.1 Identification of random amplified polymorphic DNA on the W chromosome of the Chinese 137 strains of the silkworm, Bombyx mori Abe, H.;Shimada, T.;Yokoyama, T.;Oshiki, T.;Kobayashi, M.
  2. Cytology v.1 Chromosome complex of Ussuri geographical race of Bombyx mori L Astaurov, B. L.;Garisheba, M. D.;Radinskaya, I. S.
  3. Bull. Ent. v.82 Use of random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) to detect DNA polymorphisms in aphids Black, W. C.;Duteau, N.;Putetka, G.;Nechols, J.;Pettorini, J.
  4. 蠶絲科學と技術 v.20 no.2 蠶品種の分化と類緣關係 蒲生卓磨
  5. 蠶試報 v.28 血液の蛋白質多型にもとつく蠶品種の系統發生學的 硏究 蒲生卓磨;大塚雄
  6. Insect Mol. Biol. v.2 Characterization of differences between whiteflies using RAPD-PCR Gawel, N.;Bartlett, A.
  7. Genome v.37 Resolution of populations of the Mediterranean fruit fly at the DNA level using random primers for the polymerase chain reaction Haymer, D.;Mclnnis, D.
  8. 한국유전학회지 v.17 no.4 RAPD-PCR을 이용한 누에 계통간 유연관계 분석 황재삼;이진성;강현아;이상몽;서동상
  9. 한국잠사학지 v.38 no.1 RAPD-PCR 분석을 위한 기초연구 황재삼;이진성;강현아;이상몽;서동상
  10. PCR protocols, a guide to methods and application Optimization of PCRs Innis, M. A.;Gelfand, D. H.;Sninsky, J.;White, T. S.
  11. 蠶試報 v.17 微生物定量法による絹蛋白質のアミノ酸助成および化學構造に關する硏究 桐村二郞
  12. Mol. Gen. Genet. v.203 The sequence around the 5, end of the fibroin gene from the wild silkmoth, Bombyx mandarina and comparison with that of the domesticated species, B. mori Kusuda, J.;Tazima, Y.;Onimaru, K.;Ninaki, O.;Suzuki, Y.
  13. 蠶試報 v.13 日本種, 支那種, 印度支那種及び歐羅巴種のアミラ-ゼ型の分析 松村秀美
  14. Chromosoma v.47 Cytological evidence for holocentric chromosomes of the silkworms, Bombyx mori and B. mandarina, (Bombycidae, Lepidoptera) Murakami, A.;Imai, H.
  15. NTSYS-pc Numerical taxonomy and multivariate analysis system(version 1.50) Rohlf, F. J.
  16. molecular insect science Development transition of silk gene expression in the Bombyx mori Suzuki Y. S.;Takiya, T.;Suzuki, C. C.;Hui, K.;Matsuno, M.;Fukuta, T.;Nagata, T.
  17. Jpn. J. Genet. v.4 Difference in the multiple forms of several enzymes between wild and domesticated silkworms Yoshitake, N.
  18. 遺傳雜 v.41 種タ酸素型からみたクワコとカイコの類緣關係につぃて 吉武成美
  19. 日蠶雜 v.37 家蠶日本種の起源に關する-考察 吉武成美
  20. 家蠶生化學 吉武成美