Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Genetic Analysis of the Envelope Region of Hepatitis G Vrius isolated from Korean Hepatitis Patients

한국인 간염환자에서 분리한 G형 감염바이러스(HGV)의 외피영역의 유전적 다양성 분석

  • 김종경 (부산대학교 자연과학대학 미생물학과) ;
  • 장경립 (부산대학교 자연과학대학 미생물학과)
  • Published : 1998.08.01
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Abstract

The genetic of a recently described virus, hepatitis G virus(HGV) was investigated. HGV envelope 1 (E1) nucleotide sequences isolated from six Korean hepatitis b virus-positive patients by using a reverse transcription-poly-merase chain reaction procedure, were analysed and compared to the seven previously reported HGV isolates. Sequence homology among the Korean isolates was 88-97% whereas among the isolates from different geographic areas was 80-92%, indicating geographical divergence of HGV. Nucleotide substitutions spread uniformly throughiut the E1 fragment. Furthermore, compared to the prototype HGV sequence, frameshift mutations were observed in most of the Korean isolating that a different translating initiation site for the polyprotein exists in the Korean type HGV.

Keywords

References

  1. Science v.271 Molecular cloning and disease association of hepatitis G virus : a transfusion-transmissible agent Linnen, J.;Wages, J.;Zhang-Keck, Z. Y.;Fry, K. E.;Krawczynski, K.;Alter, H.;Koonin, E.;Gallagher, M.;Alter, M.;Hadziyannis, S.;Karayiannis, P.;Fung, K.;Naskatsuji, Y.;Shih, W. K.;Young, L.;Piatak, M. Jr.;Hoover, C.;Fernandez, J.;Chen, S.;Zou, J. C.;Morris, T.;Hyams, K. C.;Ismay, S.;Lifson, J. D.;Hess, G.;Foung, S. K. H.;Thomas, H.;Bradley, D.;Margolis, H.;Kim, J. P.
  2. Biochem. Biophys. Res. Commun. v.228 Sequence of hepatitis G virus genome isolated from a Japanese patient with non-A-E-hepatitis : amplification and cloning by long reverse transcription-PCR Shao, L.;Shinzawa, H.;Ishikawa, K.;Zhang, X.;Ishibashi, M.;Misawa, H.;Yamada, N.;Togashi, H.;Takahashi, T.
  3. J. Med. Virol. v.48 Sequence and genomic organization of GBVC : a novel member of the falviviridae associated with human non-A-E hepatitis Leary, T. P.;Muerhoff, A. S.;Simons, J. N.;Pilot-Matias, T. J.;Erker, J. C.;Chalmers, M. L.;Schlauder, G. G.;Dawson, G. J.;Desai, S. M.;Mushahwar, I. K.
  4. J. Med. Virol. v.51 Infection with GB virus C in patients with chronic liver disease Sugai, Y.;Nakayama, H.;Fukuda, M.;Sawada, N.;Tanaka, T.;Tsuda, F.;Okamoto, H.;Miyakawa, Y.;Mayumi, M.
  5. J. Gen. Virol. v.73 Genetic diversity between hepatitis G virus isolates : analysis of nucleotide variation in the NS-3 and putative 'core' peptide genes Pickering, J. M.;Thomas, H. C.;Larauoammos, P.
  6. Proc. Natl. Acal. Aci. U. S. A. v.94 Cloning and characterization of the extreme 5'-terminal sequences of th RNA genomes of GB virus C/hepatitis G virus Hsieh, S. Y.;Yang, P. Y.;Chen, H. C.;Liaw, Y. F.
  7. Mol. Cell. v.3 Genetic variability in the envelope region of hepatitis C virus isolated from korean patients with Non-A, Non-B hepatitis Jang, K. L.;Cho, Y. G.;Song, M. K.;Kim, C. M .;Lee, D. S.;Sung, Y. C.
  8. Anal. Biochem. v.162 Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction Chomczynski, P.;Sacchi, N.
  9. Proc. Natl. Acad. Sci. U. S. A. v.74 DNA sequencing with chain-terminating inhibitors Sanger, F.;Nicklen, S.;Coulson, A. R.
  10. J. Gen. Virol. v.78 The entire nucleotide sequences of two GB virus C/hepatitis G virus isolates of distinct genotypes from Japan Okamoto, H.;Nakao, H.;Inoue, T.;Fukuda, M.;Kishimoto, J.;Lizuka, H.;Tsuda, F.;Miyakawa, Y.;Mayumi, M.
  11. Bull. Acad. Mil. Med. Sci. v.20 cDNA cloning and sequencing of HGV genome from chinese Zhou, Y. S.;Chen, W.;Zhao, H. L.;Zhang, J. S.;Xu, J.;Wang, H. T.
  12. J. Gen. Virol. v.77 Molecular cloning and characterization of a GB virus C isolate from a patient with non-A%E hepatitis Erker, J. C.;Simons, J. N.;Muerhoff, A. S.;Leary, T. P.;Chalmers, M. L.;Desai, S. M.;Mushahwar, I. K.
  13. J. Virol. v.70 Translation initation in GB viruses A and C : evidence for internal ribosome entry and implications for genome organization Simons, J. N.;Desai, S. M.;Schultz, D. E.;Lemon, M.;Mushahwar, I. K.