포항지역의 중금속과 탄화수소 내성균 분포

Distribution of Heavy Metals and Hydrocarbons Resistant Bacteria at Pohang Area

  • 김갑정 (한남대학교 이과대학 미생물학과) ;
  • 이인수 (한남대학교 이과대학 미생물학과) ;
  • 박경량 (한남대학교 이과대학 미생물학과)
  • 발행 : 1998.06.01

초록

As a part of a study on the development of microbiological methods for petroleum exploration, the distribution of the avarafe survival rate for heterotrophs to various heavy metal ions and hydrocarbons were surveyed and compared by the use of plate count method. In consequence of the avarage survival rate to heavy metal ions(2 hours treatment) and hydrocarbons(1 hour treatment) for heterotrophs isolatinf from soil samples(50cm depth) which located in Doum mountain(A, B and D site) and Aedowon(C site) at Pohang area, the survival rate of heterotrophs for nickel(600ppm), cobalt(500ppm), cadmiun(100ppm), mercury(20ppm), zinc(400 ppm) and lead(500ppm) were 73.7%, 82.6%, 76.8%, 9.5%, 77.8% and 73.6% at A site and 67.9%, 82.5%, 86.0%, 5.8%, 82.5% and 91.7% at B site, 87.8%, 79.8%, 87.5%, 7.0%, 84.2% AND 47.7% AT c SITE, AND 71.8%, 76%, 85.9%, 1,2%, 79.6% AND 88.3% AT D site, respectively. Also the survival rate of heterotrophs from A,B,C and D site to pentane and hexane(each concentration is 20%) were 26.7% and 42.5%, 11.8% and 8.1%, 44.3% and 36.2%, and 12% and 3.5%, respectively. therefore, heterotrophs from B and D site that alternated gravelstone, muddy sandstone and sandstone were higher survival rate to the heavy metal ions than heterotrophs from A site which mainly composed gravelstone. Also, heterotrophs from C site which mainly composed muddy sandstone and once produced natural gas were showed relatively higher survival rate to the heavy metal ions and hydrocarbons than the other sites. Consequently, we confirmed that the distributions of tolerant heterotrophs to heavy metal ions and hydrocarbons were differ from the lithological compositon.

키워드

참고문헌

  1. Introduction to environmental microbiology Mitchell, M.
  2. A Symposium under the Auspices of the Institute for the Study of Earth and Man A Critical overview and proposed working model of surface geochemical expolration Unconventional methods in exploration for petroleum and natrual gas Price, L. C.;M. J. Davidson(ed.)
  3. Appl. Environ. Microbiol. v.49 no.2 Effect of metalrich sewage sludge application on the bacterial communities grasslands Barkay, T.;Tripp S. C.;Olson B. H.
  4. Pertroleum microbiology Davis, J. B.
  5. Proc. Nat. Acad. Sci. v.91 Targeted disrupthin of metallothioneinⅠandⅡgenes increases sensitivity to cadmium Masters, B. A.;Kelly E. J.;Ouaife C. J.;Brinster R. L.;Palmitr R. D.
  6. J. Bacteriol. v.162 no.1 Alcaligenes eutrophus CH34 is a facultative chemo-lithotroph with plasmid-bound resistance to heavy metal Mergeay, M.;Nies D.;Schlegel H. D.;Gerits J.;Charles P.;Gijsegem F. V.
  7. J. Bacteriol. v.171 no.7 Metal ion uptake by a plasmid-free metal-sensitive Alcaligenes eutrophus strain Nies, D. H.;Silver S.
  8. Appl. Environ. Microbiol. v.53 no.12 Testing for bacterial resistance to arsenic in monitoring well water by the direct viable counting method Zelibor, J. L. Jr.;Doughten M. W.;Grimes D. J.;Colwel R. R.
  9. Geochemical methods of prospecting and exploration for petroleum and natural gas Kartsev, A. A.;Tabasaranskii, Z. A.;Subbota, M. I.;Mogilevskii, G. A.;P. A. Witherspoon(ed.);W. D. Romey(ed.)
  10. Oil and Gas Journal Geochemical methods of prospecting for hydrocarbons Duchscherer, W.
  11. American Association of Petroleum Geologists Bulletin v.68 Electrochmical aspects of hydrocarbon reservoirs and surface seeps Sivenas, P.;Beales, F.
  12. U.S. Geological survey Open-File Report v.44 Late diagenetic indicators of buried oil and gas Donovan, T. J.;Dalziel, M. C.
  13. 3rd International Workshop on Fan Deltas Field Excursion Guidebook Fan Deltas in the Pohang Basin(Miocene) Chough, S. K.;Choe M. Y.;Hwang, I. G.
  14. U. S. Patent, 5055397 Geomicrobiological methods of ore and petroleum exploration Michaelis, G. B.;Riese W. C.