Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Production of the Extracellular Alkaline Proteinase by Yarrowia Lipolytica 504D

Yarrowia lipolytica 504D의 Extracellular Alkaline Proteinase 생산성

  • 유춘발 (대구대학교 식품공학과) ;
  • 김창화 (대구대학교 식품공학과) ;
  • 김태곤 (대구대학교 식품공학과)
  • Published : 1998.06.01
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Abstract

Productivity of alkaline proteinase from Yarrowia lipolytica 504D was investigated. For the production fo the enzyme, hemoglobin was the best nitogen source, however, casein and skim milk were also good. All carbon sources inhibited strongly the producitivity of the enzyme. Yeast extract increased the productivity of the enzyme to 220%, but almost mineral salts except monovalant ions decreased it. Based on these results, optimal medium was composed of 1.2% casein, 0.2% glucose, 0.16% yeast extract, and 0.1% ammonium sulfate. the best condition for the production of the enzyme was observed at pH 9 and $20^{\circ}C$ for 42 hours.

Keywords

References

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