생명과학회지 (Journal of Life Science)

  • 제8권2호
  • /
  • Pages.216-225
  • /
  • 1998
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

Salmonella typhimurium의 금속이온에 대한 유전적 반응

Genetic Responses to Metal ion in Aslmonella typhimurium

  • 정주리 (한남대학교 미생물학과) ;
  • 박경량 (한남대학교 미생물학과) ;
  • 고상균 (대전대학교 미생물학과) ;
  • 박용근 (고려대학교 생명공학원) ;
  • 이인수 (한남대학교 미생물학과)
  • 발행 : 1998.04.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Metal ion-induced and it’s regulatory genes were screened in virulent salmonella typhimurium UK1 and tested cross-regulation with various stresses. Using the techniqud of P22-MudJ(Km, lacZ)-directed lacZ operon fusion, LF40 cuiA::MudJ and Lf153 cuiD::MudJ which were induced by copper were selected. cuia and cuiD were determined anaerobic coper inducible and copper tolerance response gene, respectively. Also cuiA and cuiD locus were determined at 81 and 8min, respectively, on salmonella Genetic Map. The two regulators were identified as cuaR, and cudR, which controls cuiA and cuiD, respectively. cuaR, and cudR appeared as negative regulators because the expression of cuiA-lac-Z and cuiD-lacZ were increased. Copper adapted UK1 showed high resistance to H$_{2}$O$_{2}$, but cuiD did not. The product of the cudR locus was responsible for decreasing the tolerance to copper and H$_{2}$O$_{2}$. Furthemore cuiA and cuiD locus were found to be part of a regulon under the control of a trans-acting regulators, rpoS, oxyR and relA. Therefore, the results suggest CTR participate with oxidative stress on Salmonella.

키워드

참고문헌

  1. J. of Bacteriol. v.165 Oxygen rgulated stimulons of Salmonella typhimurium identified by MudJ(Ap, laZ) operon fusions Aloabadi, Z.;Warren, F.;Mya, S.;Foster, J. W.
  2. J. of Bacteriol. v.170 Novel regulatory loci controlling oxygen and pH regulated gene expression in S. typhimurium Aliabadi, Z.;Park, Y. K.;Slonczewski, J. L.;Foster, J. W.
  3. J. of Bacteriol. v.174 Rapid mapping in Salmonella typhi with Mud-P22 prophages Benson, M. R.;Goldman, B. S.
  4. Metal ions and baceria BeVeridge, T. J.;Doyle, R. J.
  5. Metal ions in Biological systems v.30 Bacterial transport of and tesistance to copper Brown, N. L.;Lee, B. T. O.;Silver, S.;H. Sigel(ed.);A. Sigel(ed.)
  6. Plasmid v.27 Coper resistance determinants in baceria Brown, N. L.;Rouch, D. A.;Lee, B. T. O.
  7. Trands Genetics v.10 Wilson disease and menkes disease : new handles on heavy metal transport Bull, P. C.;Cox, D. W.
  8. FEMS Microbiology Letters v.11 Toxicity of heavy metals to soil bacteria Duxbury, T.
  9. Soil Biol. Biochem. v.15 Metal-tolerant bacterial populations from natural and metal-poluted soils Ducbury, T.;Bicknell, B.
  10. Moleculat Microbiology v.25 no.4 Bacterial copper- and zinc-cofactored superoxide diamutase contributes to the pathogenesis of systemic salmonellosus Farrant, J. L.;Sansone, A.;Canvin, J. R.;Pallen, M. J.;Langford, P. R.;Wallis, T. S.;Dougan, G.;Kroll, J. S.
  11. J. of Bacteriol. v.174 Effect of Salmonella typhimurium ferric uptake regulator(fur) mutation on iron-and pH-regulated protein synthesis Foster, J. W.;Hall, H. K.
  12. Microbiol. v.140 Regulatory circuits involved with pH-regulated gene expression in Salminella typhimurium Foster, J. W.;Park, Y. K.;Bang, I. S.;Katem, K.;Bettes, H.;Hall, H. K.;Shaw, E.
  13. J. of Bacteriol. v.177 Identification of cutC and cutF(nlpE) genes involved in copper tolerance in Escherichia coli Gupta, S. D.;Lee, B. T. O.;Camakatis, J.;Wu, H. W.
  14. J. of Bacteriol. v.179 A Salamonella typhimurium genetic locus which cofers copper tolerance on copper-sensitive mutants of Escherichia coli Gupta, S. D.;Wu,H. C.;Ric, P. D.
  15. J. Free. Rad. Biol. Med. v.2 The role and mechanism of metal ions and their complexed in enhancing damage in biological systems or in protecting these systems from the toxicity of )2- Gldstein, S.;Czapski, G.
  16. Ann. Rev. Genet. v.18 Bacterial Regulation ; Global regulatory networks Gorresman, S.
  17. Ann. Rev. Microbiol. v.40 Microorganisms in reclamation of metal Herchins, S. R.;Davidson, M. S.;Brierley, J. A.;Brierley, C. L.
  18. J. Bacteriol. v.152 Bacteriophage P22 as a vector for Mu mutagenesis in Salmonella typhomurium ; isolation of nad-lac and pnc-lac gene fusions Holly, E. A.;Foster, J. W.
  19. J. of Bacteriol. v.173 Fnr mutants that activate gene expression in the presence of oxygen Kiley, P. J.;Reznikoff, W. S.
  20. Kor. J. Mocrobio. v.27 A study of aerobic and anaerobic inducible genes using MudJ(Km, lacZ)opereon fusion in Salmonella typhimurium Kim, J. S.;Yoo, Y. D.;Park, J. H.;Kim, Y. K.;Lee, I. S.;Park, Y. K.
  21. Metal Speciation in the Environment Lee, B. T. O.;Brown, N. L.;Rogers, S.;Bergemann, A.;Camakaris, J.;Zouch, D. A.
  22. J. of Bacteriol. v.176 A low-pH-inducible, stationary-phase aci tolerance response in Salmonella typhimurium. Lee, I. S.;Slonczewski, J. L.;Foster, J. W.
  23. Molecular Microbiology v.17 The stationatu-phase sigma factor $δ^s$(RpoS) in required for sustained acid tolerance response in virulent Salmonella typhimurium Lee, I. S.;Lin, J.;Hall, H. K.;Bearson B.;Foster, J. W.
  24. Korean J. Envitron. Biol. v.11 Characterization and cloning of regulatory genes for anaerobic environment inducible gene expression in Salmenella typhimurium Lee, Y. J.;Bang, I. S.;Lee, I. S.;Lee, Y. N.;Park, Y. K.
  25. J. of Bacteriol. v.177 Compatative analysis of exterme acid survival in Salmenella typhimurium, Shigella flexneri, and Escherichia coli Lin, J. Lee, I. S.;Frey, J.;Slonczewski, J. L.;Foster, J. W.
  26. A Short Coures in Bacterial Genetics Miller, J. H.
  27. Metal ion homeostesis : molerculat biology and cheistry Copper transport in Escherichia coli Rouch, D.;Camakaris, J.;Lee, B. T. O.;Hamer, D. H.(ed.);Winge, D. R.(ed.)
  28. J. Biol. Chem. v.264 Site-specific oxidative in DNA damage at polyguanosines produced by copper plus hydrogen peroxide Sagripanti, J. L.;Kraemer, K. H.
  29. Mol. Microbiol. v.10 Human Menkes X-chromosome disease and the staphylococcal cadmium-resistance ATPase ; a remarkable similarity in protein sequences Silver, S.;Nucifora, G.;Phung, L. T.
  30. J. of Bacteriol. v.168 Global control in Samonella typhimurium ; Two dimensional electrophoretic analysis of starvation, anaerobiosis, and heat shock inductive proteins Spector, J.;Aliabadi, Z.;Gonzalez, T.;Foster, J. W.
  31. J. Bio. Chem. v.267 Binding of the metalloregulatory protein Dtx to the diptheria tox operator requires a divalent heavy metal ion and prtect the palindromic sequence from DNase I digestion Tao-X;Murphy J. R.
  32. J. Bio. Chem. v.268 Regulation of metallothionein genes by the ACR1 and AMT1 teanscription factor Thorvaldsen, J. L.;Sewell, A. K.;McCowen, C. L.;Winge, D. R.
  33. J. Bio. Chem. v.266 Mutation spectrum of copper-induced DNA damage Tkeshelashvili, L. K.;McBride, T.;Spence, K.;Loeb, L. A.
  34. Mutation Res. v.389 Intracellular generation of superoxide by copper sulphate in Escherchia coli Tsuyoshi, K.;Hajime, N.
  35. J. Bio. Chem. v.93 Acetylornithase of Escherichia coli ; partial purification and some properties Vogel, H. J.;Bonner, D. M.
  36. Genetics v.118 Packaging specific segments of the Salmunella chromosome with lacked in Mud-P22 prophages Youderian, P.;Braier, P.;Sugino, K. L.;Higgins, N. P.;Elliot, T.