Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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PCR and Restriction Fragment Pattern of 16S rRNA gene of Vibrio vulnificus

Vibrio vulnificus ATCC 27562의 16S rRNA 유전자의 PCR과 제한효소절단 방식

  • 허문수 (국립수산진흥원 증식부 병리과) ;
  • 정초록 (부산대학교 대학원 미생물학과)
  • Published : 1998.04.01
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Abstract

A pair of designed primers (sequences from Gene Bank) amplified 16S fRNA gene of V. vulnificus within polymerase chain reaction (PCR) machine. This PCR product is about 1.3kb DNA fragment. Six enzymes (BamH I, Alu I, Sau3A I, Hind III, Sal I, Sma I) were used for restriction pattern analysis of amplified 16S rRNA gene of V. vulnificus ATCC 27562. Digested fragments are resolved by 3% agarose gel. BamH I did not show digested fragment so, there was no cutting site of BamH I in PCR product. Alu I produced three small fragments from 400 bp to 200 bp. Sau3A I produced three fragments larger than Alu I from 70 bp and 500 bp. One of fragments of Sal I was same with 500 bp of Hind III fragment and the other was 750 bp. Sma I showed two fragments of 800 bp and 470 bp. The profile of digested fragments of 16S rRNA of V.vulnificus ATCC 27562 will may be able to use standard profile for identification of V. vulnificus.

Vibrio unlnificus ATCC 27562의 16S rRNA 유전자를 PCR법과 제한효소절단법으로 분석 하여 얻은 결과는 다음과 같다. 1. 고안된 한쌍의 primer로 PCR을 시행하여 얻은 산물은 약 1.3Kb 였다. 2. PCR산물을 여섯가지의 제한 효소로 절단하여 얻은 단편들은 아래와 같다. BamH I: 어떤 restriction fragment도 만들지 않았다. Alu I : 약 400bp와 200bp의 두가지 fragment를 생산하였다. Sau3A I : 약 70bp에서 450bp 사이에 세가지 fragment를 생산하였다. Hind III : 약 800bp와 500bp의 약간 큰 두가지 fragment를 생산하였다. Sal I : 약 500bp와 750bp의 두가지 fragment를 생산하였다. Sma I : 약 800bp와 470bp의 두가지 fragment를 생산하였다.

Keywords

References

  1. Appl. Environ. Microbiol. v.60 Pcr and restrictio fragment length polymorphism of pelgene as a tool to identify Erwinia carorovora in relation to potato disease Armelle, D.;Sylvie, P.;Alain, K.;YVES, B.
  2. Disease of Crustacea, Disease caused by microorganisms in animals BOCK, J. A.;Lightner, D. V.
  3. Identification of bacterial pathology by PCT-RFLPs JIE, K.
  4. Int. J. Sys. Bac. v.43 Phylogenetic relationship of marine bacteria mainly members of the family Vibrionacea determined on the basis of 16s RNA sequences KUMIKO KITA-TSUKAMOTO;HIROSHIOYAIZU;KENJI NANBA;USIO SIMDU
  5. FEMS Microbiol. lett. v.124 Discrimination of Myconacterium aviumm-Mycobacterium intracellular strain by genomic DNA fingerprinting with 16s RNA gene probe RACHEL Peillon;Emmanual B. Drouet;Sylvie Bruneau;Gilles Panteix;Gerard Antoine;Denoyel;Henri P. De Montclos
  6. J. Bac. v.172 Rapid genetic identofication and mapping of enzymically amplified ribosomal DNA from several Cryptococcus species RYTAS VILGALYS;MARK HESTER