Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Establishment of Transgenic Drosophila Bearing Metallothionein-lacZ Fusion Gene and Its Practical Use

  • Kim, Young-Shin (Department of Biology, College of Natural Sciences, Pusan National University, Pusan 609-735) ;
  • Park, Hyun-Sook (Department of Molecular Biology, College of Natural Sciences, Pusan National University, Pusan 609-735) ;
  • Yoo, Mi-Ae
  • Published : 1998.06.01
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Abstract

The reporter plasmid pMT-lacZ containing the metallothionein (MT) promoter region (-320∼+58 with respect to the transcription initiation site) fused to the lacZ gene in a P-element vector was constructed. Transgenic Drosophila bearing the MT-lacZ fusion gene were established by P-element mediated transformation. Expression of the MT-lacZ fusion gene in transformants was examined during development. By treatment with low concentration of cadmium (>1O uM) or paraquat (>50 uM), increased expression of B-galactosidase was shown in fat body, brain lobe, and ganglion transgenic larval tissues. The results show that transformants bearing the MT-lacZ fusion gene are useful for further studies on the mechanism of regulation of MT gene expression and for monitoring toxic metals.

Keywords

References

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