Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Characteristics of $[^3H]$-Choline Uptake into Synaptosomes from Rat Hippocampus

  • Lee, Se-Eun (Department of Physiology, College of Medicine, Korea University, Department of Biology, College of Natural Science, Yonsei University) ;
  • Hong, Seong-Gil (Department of Physiology, College of Medicine, Korea University) ;
  • Choe, Rim-Sun (Department of Biology, College of Natural Science, Yonsei University)
  • Published : 1998.06.01
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Abstract

Certain basic characteristics of choline uptake in nerve terminals were studied with synaptosomes from rat hippocampus. Synaptosomal $[^3H]$-choline uptake was clarified as specific and high affinity by low Km value(2.2 uM), Na+-dependency and high sensitivity to hemicholinium-3, a competitive inhibitor of choline uptake. Choline uptake into synaptosomes was linearlys related to Na+ concentration and membrane potential. Extracellular Ca2+ modulated the choline uptake, but probably not through increase of intracellular $Ca^{2+}$, because this modulation was not affected the by high $K^+$-depolarization. EGTA (2mM) added for $Ca^{2+}$-free condition had a peculiar effect of decreasing choline uptake. These results suggest that Ca2+ may play an important role in regulating the metabolism of acetylcholine in the nerve terminals directly through the increase of acetylcholine release.

Keywords

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